Genetic factors determining varietal differences in characters affecting yield between two rice (Oryza sativa L.) varieties, Koshihikari and IR64
Genetic factors determining varietal differences in characters affecting yield between two rice (Oryza sativa L.) varieties, Koshihikari and IR64
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DOI：
10.1007/s10722-015-0237-3
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发表时间：
2016-01-01
影响因子：
2
通讯作者：
Ishimaru, Ken
中科院分区：
文献类型：
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作者：
Ujiie, Kazuhiro;Yamamoto, Toshio;Ishimaru, Ken
日本优质水稻品种Koshihikari的产量相对低于现代高产品种。IR64携带几个众所周知的基因，如控制籽粒大小的重要基因GS3，半矮秆基因SD-1，以及控制小而窄的旗叶的窄LEAF1(NAL1)基因。本研究利用来自Koshihikari和IR64的两组染色体片段置换系(CSSL)，试图通过分析影响一个性状的染色体区域(CRATS)的功能来评估导致亲本差异的遗传因素。对于28个性状，我们在Koshihikari背景下鉴定出312个克拉，在IR64背景下鉴定出275个克拉。其中供体等位基因对84个克拉和103个克拉产生正效应。其中，与GS3相关的CRAT和与粒数相关的CRAT扩大了Koshihikari的潜在库容，但这并不影响最终产量。组合克拉特可以增强来源能力，可能会增加粮食产量。虽然sd-1基因可以提高水稻的抗倒性，但sd-1基因的CSSL产量下降了28.7%。这些结果表明，SD-1提供的较小生物量可能会降低冠层光合作用。在Koshihikari背景中，与NAL1相关的CRAT和位于CHR上的CRAT。6提高了SPAD值，但对叶片大小的影响相反。在CHR上检测到的两个板条箱。6和7增加了叶面积，但对SPAD值没有影响。这些克拉特的面积和SPAD值的组合可能会提高来源能力。
The yield of Koshihikari, a Japanese premium rice variety, is relatively lower than that of modern high yielding varieties. IR64 carries several well-known genes such as GS3, an important gene for grain size, sd-1, a semi-dwarf gene, and NARROW LEAF1 (NAL1), a gene for small, narrow flag leaves. In this study, we used two sets of chromosome segment substitution lines (CSSLs), from Koshihikari and IR64, and attempted to evaluate the genetic factors that cause differences between parents by analyzing the function of chromosome regions affecting a trait (CRATs). For 28 traits, we identified 312 CRATs in the Koshihikari background and 275 in the IR64 background. In these, donor alleles had positive effects in 84 and 103 CRATs, respectively. Among these, the CRAT related to GS3 and those for grain number expanded the potential sink size in Koshihikari, although this did not affect final yield. The combination of CRATs that enhances source ability may increase grain yield. Although the sd-1 gene might improve resistance to lodging, the yield of CSSLs with sd-1 decreased by 28.7 %. These results suggest that the smaller biomass conferred by sd-1 might reduce canopy photosynthesis. In the Koshihikari background, the CRAT related to NAL1 and those located on chr. 6 increased SPAD value but had the opposite effect on leaf size. Two CRATs that were detected on chr. 6 and 7 increased leaf area without any effect on the SPAD value. The combination of these CRATs for area and SPAD value might improve source ability.