The development of inhibitory synaptic specializations in the mouse deep cerebellar nuclei
The development of inhibitory synaptic specializations in the mouse deep cerebellar nuclei
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DOI：
10.1016/s0306-4522(01)00127-0
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Escher, G
中科院分区：
文献类型：
--
作者：
Garin, N;Escher, G
应用激光共聚焦扫描显微镜和免疫组织化学方法，观察到小鼠小脑深部核团神经元与浦肯野细胞之间GABA能突触的完整形态发育。首先，在突触后GABAA受体尚未聚集在细胞膜上但胞浆呈弥漫性分布时，用抗突触素、突触素或谷氨酸脱羧酶的抗体检测到突触前曲张，这表明突触前终末比靶细胞成熟。其次，在最初一周的扩散表达后，受体聚集体突然发展起来，这些聚集体成熟为更多更大的突触聚集体。在突触后成熟过程中，突触前曲张形成许多清晰的斑点。一旦检测到突触前和突触后的簇，这些站点总是共存的。因此，我们认为突触后受体在发育过程中的聚集是突触形成的一个标志。我们的观察结果与突触前神经元在靶神经元表达其受体在膜上成熟之前分化轴突的发育模型是一致的。因此，突触前神经元可以指示靶神经元突触后受体在突触的分布和聚集。(C)2001年IBRO。爱思唯尔科学有限公司出版。版权所有。
Using confocal laser scanning microscopy and immunohistochemistry, this study shows the complete morphological development of GABAergic synaptic contacts between Purkinje cells and neurons of the deep cerebellar nuclei of the mouse. Firstly, presynaptic varicosities visualized with antibodies against synaptophysin, synapsin or glutamic acid decarboxylase, were detected when the postsynaptic GABAA receptors were not yet aggregated in the membrane but had a diffuse cytoplasmic distribution, which indicated a lead in maturation of presynaptic terminals over target cells. Secondly, receptor aggregates developed suddenly after an initial week of diffuse expression and these clusters matured into more numerous and larger synaptic aggregates. During this postsynaptic maturation, the presynaptic varicosities develop into numerous and well-defined spots. As soon as both pre- and postsynaptic clusters were detectable, these sites are always colocalized. We therefore consider the aggregation of postsynaptic; receptor during development as a landmark of synapse formation.Our observations are consistent with a developmental model in which the presynaptic neuron differentiates its axon before the target neuron expresses the mature form of its receptors on the membrane. The presynaptic neuron can therefore instruct the target neuron about the distribution and aggregation of the postsynaptic receptors at the synapse. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.