PERSISTENT ACTIVATION OF THE ZETA-ISOFORM OF PROTEIN-KINASE-C IN THE MAINTENANCE OF LONG-TERM POTENTIATION
PERSISTENT ACTIVATION OF THE ZETA-ISOFORM OF PROTEIN-KINASE-C IN THE MAINTENANCE OF LONG-TERM POTENTIATION
复制标题
DOI：
10.1073/pnas.90.18.8342
复制
发表时间：
1993-09-15
影响因子：
11.1
通讯作者：
SUBLETTE, E
中科院分区：
文献类型：
--
作者：
SACKTOR, TC;OSTEN, P;SUBLETTE, E
海马CA1区的长时程增强是大脑中记忆形成的模型，分为两个阶段。一个短暂的过程（诱导）被启动，然后产生一个持久的机制（维持），以增强突触强度。蛋白激酶C（PKC）是一个由多种同工酶组成的基因家族，可能在诱导和维持中发挥作用。在大鼠海马脑片CA1区，大多数PKC同工酶易位到颗粒级分破伤风后15秒。颗粒组分中PKC的增加并没有持续到长时程增强的维持阶段。与此相反，组成型活性激酶，PKM，一种形式的特定于一个单一的同工酶（zeta），在维持阶段的细胞质中增加。从PKC转位到PKM形成的转变可能有助于解释长时程增强的诱导和维持的分子机制。
Long-term potentiation in the CA1 region of the hippocampus, a model for memory formation in the brain, is divided into two phases. A transient process (induction) is initiated, which then generates a persistent mechanism (maintenance) for enhancing synaptic strength. Protein kinase C (PKC), a gene family of multiple isozymes, may play a role in both induction and maintenance. In region CA1 from rat hippocampal slices, most of the isozymes of PKC translocated to the particulate fraction 15 sec after a tetanus. The increase of PKC in the particulate fraction did not persist into the maintenance phase of long-term potentiation. In contrast, a constitutively active kinase, PKM, a form specific to a single isozyme (zeta), increased in the cytosol during the maintenance phase. The transition from translocation of PKC to formation of PKM may help to explain the molecular mechanisms of induction and maintenance of long-term potentiation.