Conservation and divergence of known apicomplexan transcriptional regulons.
Conservation and divergence of known apicomplexan transcriptional regulons.
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DOI：
10.1186/1471-2164-11-147
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发表时间：
2010-03-03
期刊：
影响因子：
4.4
通讯作者：
Stoeckert, Christian J Jr
中科院分区：
文献类型：
--
作者：
Essien, Kobby;Stoeckert, Christian J Jr
背景技术背景：顶复门是一个不同的寄生虫门，引起各种疾病，包括各种动物的疟疾和牛的淋巴细胞增生。关于这些不同的寄生虫如何调节它们的转录调节子，我们知之甚少。更少的是知道如何调节子系统，包括转录因子和靶基因连同其相关的生物过程，在这些不同的parasitis.RESULTS：为了深入了解这些寄生虫之间的转录调控的差异，我们比较了假定的疟疾转录因子跨物种的同源性配置文件，并检查了富集模式的4个结合位点在11 apicomplexans。大约五分之三的因子是广泛保守的几个系统发育顺序的测序apicomplexans。这一观察结果表明存在调节子，其调节在这个古老的门中是保守的。在整个门中不广泛保守的转录因子可能参与调节子系统，这些调节子系统在物种之间已经分化。根据转录因子保守模式检查结合位点富集模式提示了调节子系统可能发散的第二种模式-以特定方式重新连接现有的转录因子及其相关的结合位点。整合结合位点与转录因子的保守模式也促进了预测的假定监管机构的一个bindingsite.CONCLUSIONS：即使转录因子是在apicomplexans代表不足，这些因素及其相关的调节子的分布反映了共同的和家庭特异性的转录调控过程。
BACKGROUND: The apicomplexans are a diverse phylum of parasites causing an assortment of diseases including malaria in a wide variety of animals and lymphoproliferation in cattle. Little is known about how these varied parasites regulate their transcriptional regulons. Even less is known about how regulon systems, consisting of transcription factors and target genes together with their associated biological process, evolve in these diverse parasites.RESULTS: In order to obtain insights into the differences in transcriptional regulation between these parasites we compared the orthology profiles of putative malaria transcription factors across species and examined the enrichment patterns of four binding sites across eleven apicomplexans. About three-fifths of the factors are broadly conserved in several phylogenetic orders of sequenced apicomplexans. This observation suggests the existence of regulons whose regulation is conserved across this ancient phylum. Transcription factors not broadly conserved across the phylum are possibly involved in regulon systems that have diverged between species. Examining binding site enrichment patterns in light of transcription factor conservation patterns suggests a second mode via which regulon systems may diverge - rewiring of existing transcription factors and their associated binding sites in specific ways. Integrating binding sites with transcription factor conservation patterns also facilitated prediction of putative regulators for one of the binding sites.CONCLUSIONS: Even though transcription factors are underrepresented in apicomplexans, the distribution of these factors and their associated regulons reflect common and family-specific transcriptional regulatory processes.