Serum granulocyte colony-stimulating factor levels in healthy volunteers and patients with various disorders as estimated by enzyme immunoassay.
Serum granulocyte colony-stimulating factor levels in healthy volunteers and patients with various disorders as estimated by enzyme immunoassay.
复制标题
通过酶免疫测定法估计健康志愿者和患有各种疾病的患者的血清粒细胞集落刺激因子水平。
作者：
K. Watari;S. Asano;N. Shirafuji;H. Kodo;K. Ozawa;F. Takaku;S. Kamachi
为了更好地了解粒细胞集落刺激因子(G-CSF)的病理生理作用，我们用一种新开发的酶免疫测定法(Motojima等)对正常人和各种疾病患者的血清G-CSF水平进行了测定。在56名正常健康人中，有49人(88%)的水平低于该方法的灵敏度(<30pg/mL)，而其余7名健康人的水平在33-163pg/ml之间。另一方面，11例特发性再生障碍性贫血(AA)患者中9例(82%)，1例Fanconi‘s贫血患者(1例)，12例骨髓增生异常综合征(MDS)患者中6例(50%)，12例血液中无原始细胞的急性白血病患者中5例(42%)(M4：1，M5：1，L1：1，L2：2)，18例慢性髓系白血病(CML)中6例(33%)，2例慢性淋巴细胞性白血病(CLL)1例，4例肺癌患者中2例，1例周期性中性粒细胞减少患者，7例恶性淋巴瘤患者中2例4例急性感染患者的G-CSF水平从46pg/ml到>2000pg/ml不等。有趣的是，再生障碍性贫血患者血中中性粒细胞计数与血清G-CSF水平呈明显负相关(r=-8169，P<0.01)。此外，还发现3例白血病患者在中性粒细胞减少期、化疗或骨髓移植(BMT)后G-CSF水平升高，某些感染性疾病和肺癌患者G-CSF水平升高与中性粒细胞计数呈正相关。本研究未对循环G-CSF的细胞来源及其产生和分泌机制进行研究，但本文提供的数据有力地表明G-CSF作为一种循环中性粒细胞生成素发挥着重要作用。
In order to better understand the patho-physiologic role of granulocyte colony-stimulating factor (G-CSF), we estimated its serum levels in healthy persons and patients with various disorders, using a newly developed enzyme immunoassay (Motojima et al). In 49 of 56 normal healthy persons (88%), the levels were beneath the sensitivity of the assay (less than 30 pg/mL), while in the remaining seven healthy persons, the levels ranged from 33 to 163 pg/mL. On the other hand, nine of 11 patients (82%) with idiopathic aplastic anemia (AA), one patient with Fanconi's anemia, six of 12 patients (50%) with myelodysplastic syndrome (MDS), five of 12 patients (42%) with acute leukemia without any blast cells in the blood (M4: one, M5: one, L1: one, and L2: two), six of 18 patients (33%) with chronic myeloid leukemia (CML), one of two patients with chronic lymphoid leukemia (CLL), two of four patients with lung cancer, one patient with cyclic neutropenia, two of seven patients with malignant lymphoma, and four patients with acute infection had G-CSF levels ranging from 46 pg/mL to greater than 2,000 pg/mL. Interestingly, a reverse correlation between blood neutrophil count and serum G-CSF level was clearly demonstrated for aplastic anemia (r = -.8169, P less than .01). Moreover, it was found that the G-CSF level rose during the neutropenic phase of cyclic neutropenia and after chemotherapy or bone marrow transplantation (BMT) in three patients with leukemia; also high G-CSF levels were positively correlated to blood neutrophil counts in some cases of infectious disorders and lung cancer. The cellular sources and the mechanisms for production and secretion of circulating G-CSF were not investigated in this study, but the data presented here strongly indicate that G-CSF plays an important role as a circulating neutrophilopoietin.
纯化人骨髓祖细胞增殖的诱导：粒细胞集落刺激因子的快速测定。
影响因子：
20.3
作者：
Griffin,JD;Sullivan,R;Beveridge,RP;Larcom,P;Schlossman,SF
通讯作者：
Schlossman,SF
影响因子：
56.9
作者：
SOUZA, LM;BOONE, TC;WELTE, K
通讯作者：
WELTE, K