Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress.
Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress.
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对凡纳滨对虾肌肉组织的转录组分析首次揭示了对低温应激的反应
DOI：
10.1371/journal.pone.0178604
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Hu C
中科院分区：
文献类型：
--
作者：
Huang W;Ren C;Li H;Huo D;Wang Y;Jiang X;Tian Y;Luo P;Chen T;Hu C
凡纳滨对虾（Litopenaeus vannamei）是世界上重要的养殖甲壳类动物。然而，对该物种参与冷胁迫反应的分子机制知之甚少。本研究在13°C胁迫和对照温度下分别获得了4个单独的vanannamei RNA-Seq文库。共有29662个Unigenes和19619个注释基因。对4个文库进行了3次比较，获得了前20%的差异表达基因中的72个，捞出了15条GO和5条KEGG温度敏感通路。催化活性（GO: 0003824）和代谢途径（ko01100）分别是响应冷胁迫中注释最多的GO和KEGG途径。此外，还对钙、MAPK级联、转录因子和丝氨酸/苏氨酸-蛋白激酶信号通路进行了筛选和聚类。丝氨酸/苏氨酸-蛋白激酶信号通路可能在冷适应中发挥更重要的作用，而其他三条信号通路的转录量并不大。我们的结果总结了差异表达基因，并提出了主要的重要信号通路和相关基因。这些发现提供了对L. vannamei对冷胁迫反应的分子基础的第一个概要见解。
The Pacific white shrimp (Litopenaeus vannamei) is an important cultured crustacean species worldwide. However, little is known about the molecular mechanism of this species involved in the response to cold stress. In this study, four separate RNA-Seq libraries of L. vannamei were generated from 13°C stress and control temperature. Total 29,662 of Unigenes and overall of 19,619 annotated genes were obtained. Three comparisons were carried out among the four libraries, in which 72 of the top 20% of differentially-expressed genes were obtained, 15 GO and 5 KEGG temperature-sensitive pathways were fished out. Catalytic activity (GO: 0003824) and Metabolic pathways (ko01100) were the most annotated GO and KEGG pathways in response to cold stress, respectively. In addition, Calcium, MAPK cascade, Transcription factor and Serine/threonine-protein kinase signal pathway were picked out and clustered. Serine/threonine-protein kinase signal pathway might play more important roles in cold adaptation, while other three signal pathway were not widely transcribed. Our results had summarized the differentially-expressed genes and suggested the major important signaling pathways and related genes. These findings provide the first profile insight into the molecular basis of L. vannamei response to cold stress.