Expression of mRNA for brain-derived neurotrophic factor in the dorsal root ganglion following peripheral inflammation
Expression of mRNA for brain-derived neurotrophic factor in the dorsal root ganglion following peripheral inflammation
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DOI：
10.1016/s0006-8993(97)00048-6
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发表时间：
1997-02-28
期刊：
影响因子：
2.9
通讯作者：
Chu, MY
中科院分区：
文献类型：
--
作者：
Cho, HJ;Kim, SY;Chu, MY
神经生长因子（nerve growth factor，NGF）是炎症和痛觉过敏之间的联系。最近的实验表明，全身注射神经生长因子显着刺激脑源性神经营养因子（BDNF）mRNA在背根神经节（DRG）的表达。本研究应用原位杂交组织化学方法，观察了大鼠外周炎症后背根神经节BDNF mRNA的变化，并观察了BDNF和trkA mRNA的共定位。通过足底注射弗氏佐剂到爪中产生的外周组织炎症显著增加DRG中的BDNF mRNA水平，并且许多表达trkA mRNA的神经元显示BDNF mRNA的表达增加。足底注射NGF抗体和弗氏佐剂可抑制BDNF mRNA的表达。这些结果表明，外周炎症诱导的BDNF mRNA的表达增加，这是由NGF介导的DRG。(C)1997年Elsevier Science B.V.
It is well known that the nerve growth factor (NGF) may serve as a link between inflammation and hyperalgesia. Recent experiments showed that systemic injection of NGF dramatically stimulated the expression of brain-derived neurotrophic factor (BDNF) mRNA in the dorsal root ganglion (DRG). In the present study, we evaluated the change of BDNF mRNA in the DRG following peripheral inflammation and also observed colocalization of BDNF and trkA mRNAs by means of in situ hybridization histochemistry in rats. Peripheral tissue inflammation produced by an intraplantar injection of Freund's adjuvant into the paws significantly increased BDNF mRNA levels in the DRG and many neurons expressing trkA mRNA showed increased expession of BDNF mRNA. Intraplantar injection of antibody to NGF together with Freund's adjuvant prevented the increase in BDNF mRNA. These findings suggest that peripheral inflammation induces an increased expression of BDNF mRNA which is mediated by NGF in DRG. (C) 1997 Elsevier Science B.V.