Predatory efficacy of five locally available copepods on Aedes larvae under laboratory settings: An approach towards bio-control of dengue in Sri Lanka
Predatory efficacy of five locally available copepods on Aedes larvae under laboratory settings: An approach towards bio-control of dengue in Sri Lanka
复制标题
实验室环境下五种当地桡足类对伊蚊幼虫的捕食功效：斯里兰卡登革热生物防治方法
作者：
L. Udayanga;T. Ranathunge;M.C.M. Iqbal;W. Abeyewickreme;M. Hapugoda
由于不可否认的化学和机械媒介控制方法效率低下，许多国家正在寻找更有效和可持续的方法来控制登革热媒介。桡足类生物控制病媒是利用自然生态系统中的相互作用进行病媒管理的理想方法，对环境的影响最小。本研究在实验室条件下测定了当地丰富的五种桡足动物对伊蚊幼虫的捕食效果。在Gampaha和Kandy地区预先确定的地点采集桡足类，并进行形态鉴定。以草履虫为培养物，以麦粒为辅食，将桡足类单种保持为独立的群落。每个物种5只成年桡足类，分别装入200只埃及伊蚊幼虫（1龄）的容器中。在24小时内，每隔3小时对容器内存活的幼虫数进行计数。每个实验重复5次。Ae也遵循了同样的程序。蚊。利用一般线性模型（GLM）评估捕食率变化的显著性，然后在SPSS（版本23）中进行Tukey的成对比较。各桡足类昆虫的捕食效率差异显著（p<0.05），其中白足螨的捕食效率最高，棘足螨次之，而叶蝉的捕食效率最低。不同伊蚊幼虫种类对桡足类捕食率的影响显著（p<0.05），但对捕食效率的影响不显著。基于这些发现，leuckarti米氏体和scrassus米氏体具有最高的捕食效率，可推荐作为斯里兰卡伊蚊媒介生物控制的潜在候选者。
Many countries are in search of more effective and sustainable methods for controlling dengue vectors, due to undeniable inefficiencies in chemical and mechanical vector control methods. Bio-control of vectors by copepods is an ideal method of using interactions in the natural ecosystem for vector management, with minimum consequences on the environment. Current study determined the predatory efficacy of five locally abundant copepod species on, Aedes larvae under laboratory conditions. Copepods were collected from the pre-identified locations within the districts of Gampaha and Kandy, and identified morphologically. Individual species of copepods were maintained as separate colonies with Paramecium culture and wheat grain as supplementary food. Five adult copepods of each species was introduced into separate containers with 200 larvae (1st instar) of Aedes aegypti. Number of larvae survived in containers were enumerated at 3 hour intervals within a duration of 24 hours. Each experiment was repeated five times. The same procedure was followed for Ae. albopictus. Significance in the variations among predation rates was evaluated with General Linear Modelling (GLM) followed by Tukey’s pair-wise comparison in SPSS (version 23). Significant variations in predation rates of studied copepod species were reported (p<0.05), whereby M. leuckarti indicated the highest followed by M. scrassus, while C. languides indicated the lowest predatory efficacy. The effect of different Aedes larval species on the predation rates of copepods remained significant (p<0.05), even though the effect on predatory efficiency was not significant. Based on the findings, both M. leuckarti and M. scrassus, with the highest predatory efficiencies, could be recommended as potential candidates for biological controlling of Aedes vectors in Sri Lanka.
DOI：
10.4269/ajtmh.2005.72.209
发表时间：
2005-02-01
影响因子：
3.3
作者：
Harrington, LC;Scott, TW;Edman, JD
通讯作者：
Edman, JD