Brain receptor autoradiography with [3H]-YM 09151-2: a ligand for labeling dopamine D-2 receptors.
Brain receptor autoradiography with [3H]-YM 09151-2: a ligand for labeling dopamine D-2 receptors.
复制标题
使用 [3H]-YM 09151-2 进行脑受体放射自显影：标记多巴胺 D-2 受体的配体。
DOI：
10.1016/0024-3205(90)90279-z
复制
发表时间：
1990
期刊：
影响因子：
6.1
通讯作者：
Dillon,B
中科院分区：
文献类型：
--
作者：
Unis,AS;Vincent,JG;Dillon,B
应用体外受体放射自显影技术，在F344大鼠脑组织切片上，研究了[~3H]-YM-09151-2作为标记D-2受体配体的适宜性。特异性结合的[H]-ym-09151-2占总结合配体的70-80%，孵育60-90分钟后达到平衡。Scatchard分析显示Kd为626 pm表观Bmax为23.2fmol/组织切片。放射自显影显示纹状体和嗅结节内颗粒密度较高。皮质中也观察到弥漫性特异性结合。
Using the technique of in vitro receptor autoradiography to slide-mounted tissue sections, we studied the suitability of [3 H]-YM-09151-2 as a ligand for labeling D-2 receptors in adult F344 rat brains. Specific [3 H]-YM-09151-2 binding accounted for 70–80% of the total bound ligand and reached equilibrium after a 60–90 minute incubation. Scatchard analysis revealed a K d of 626 pM. The apparent B max was 23.2 fmol/tissue section. Autoradiographs demonstrated high grain densities in the striatum and olfactory tubercle. Diffuse specific binding was also observed in the cortex.