Insulin stimulation of protein synthesis in cultured skeletal and cardiac muscle cells.
Insulin stimulation of protein synthesis in cultured skeletal and cardiac muscle cells.
复制标题
胰岛素刺激培养的骨骼肌和心肌细胞中的蛋白质合成。
DOI：
10.1152/ajpcell.1982.243.1.c81
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Low,RB
中科院分区：
文献类型：
--
作者：
Airhart,J;Arnold,JA;Stirewalt,WS;Low,RB
在鸡胚心脏和骨骼肌细胞的原代分化培养物中，研究了急性暴露于胰岛素对蛋白质合成的影响。合成率计算使用tRNA结合的亮氨酸作为前体的比活性，比活性显著低于细胞外亮氨酸，但大于游离的，细胞内亮氨酸在0.2 mM的外部亮氨酸。胰岛素没有改变这些关系。在生理范围内的胰岛素剂量在两种细胞类型中产生显著的蛋白质合成刺激。在较高的胰岛素浓度下观察到最大反应，包括绝对和分数速率增加约30%。胰岛素治疗仅1小时后，在心肌细胞中观察到胰岛素的显著刺激，并且在培养基中存在和不存在血清的情况下观察到激素的作用。
The effects of acute exposure to insulin on protein synthesis were examined in primary, differentiated cultures of embryonic chick heart and skeletal muscle cells. Synthetic rates were calculated using the specific activity of tRNA-bound leucine as precursor, a specific activity that was significantly less than that of extracellular leucine but greater than that of free, intracellular leucine at 0.2 mM external leucine. Insulin did not alter these relationships. Doses of insulin in the physiological range produced significant stimulation of protein synthesis in both cell types. Maximal responses, involving approximately 30% increases in both absolute and fractional rates, were observed at higher insulin concentrations. Significant stimulation by insulin was seen in cardiac cells after only 1 h of insulin treatment, and the effects of the hormone were observed both in the presence and absence of serum in the culture medium.