Doubly linked, A-type proanthocyanidin turner and other constituents of Ixora coccinea leaves and their antioxidant and antibacterial properties
Doubly linked, A-type proanthocyanidin turner and other constituents of Ixora coccinea leaves and their antioxidant and antibacterial properties
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DOI：
10.1016/j.phytochem.2010.08.018
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发表时间：
2010-12-01
期刊：
影响因子：
3.8
通讯作者：
Abegaz, Berhanu Molla
中科院分区：
文献类型：
--
作者：
Idowu, Thomas Oyebode;Ogundaini, Abiodun Oguntuga;Abegaz, Berhanu Molla
对龙船花叶甲醇提取物乙酸乙酯部分进行植物化学研究，分离并鉴定了A型三聚体原花青素表儿茶素（2 β-> O -> 7 4 β-> 8）-表儿茶素-（5 -> O -> 2 β 6 -> 4 β）-表儿茶素，命名为ixoratannin A-2，与七种已知化合物表儿茶素矢车菊苷配质A2肉桂单宁B-1和四种黄酮-3-一起沿着醇鼠李糖苷，即山奈酚-7-O-α-L-鼠李糖苷山奈酚-3-O-α-L-鼠李糖苷槲皮素-3-O-α-L-吡喃鼠李糖苷和山奈酚-3-7-O-α-L-二鼠李糖苷UV MS 1D-和2D-NMR光谱分析并通过与文献数据比较，分离的化合物的抗氧化剂评价显示，苦皮藤单宁A-2和肉桂单宁B-1是DPPH抑制脂质过氧化和一氧化氮自由基清除试验中最具活性的化合物。通过琼脂扩散试验，使用大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和枯草芽孢杆菌评估抗菌活性。所有测试化合物抑制枯草B的生长，而只有表儿茶素和槲皮素-3-O-α-L-吡喃鼠李糖苷抑制大肠杆菌的生长（C）。2010爱思唯尔有限公司版权所有
Phytochemical investigation of the ethyl acetate fraction of the methanol extract of the leaves of Ixora coccinea led to the isolation and identification of an A-type trimeric proanthocyanidin epicatechin(2 beta -> O -> 7 4 beta -> 8)-epicatechin-(5 -> O -> 2 beta 6 -> 4 beta)-epicatechin named ixoratannin A-2 along with seven known compounds epicatechin procyanidin A2 cinnamtannin B-1 and four flavon-3-ol rhamnosides viz viz kaempferol-7-O-alpha-L-rhamnnoside kaempferol-3-O-alpha-L-rhamnoside quercetin-3-O-alpha-L-rhamnopyranoside and kaempferol-3 7-O-alpha-L-dirhamnoside The structures were elucidated by the application of IR, UV MS 1D- and 2D-NMR spectroscopic analyses and by comparison with literature data Antioxidant evaluation of Isolated compounds revealed that ixoratannin A-2 and cinnamtannin B-1 were the most active compounds in DPPH inhibition of lipid peroxidation and nitric oxide radical scavenging assays Antibacterial activities were assessed by means of agar-diffusion assays using Escherichia colt Staphylococcus aureus Pseudomonas aeruginosa and Bacillus subtilis All tested compounds inhibited the growth of B subtilis while only epicatechin and quercetin-3-O-alpha-L-rhamnopyranoside inhibited the growth of E colt (C) 2010 Elsevier Ltd All rights reserved