Development of noradrenergic neurons in the zebrafish hindbrain requires BMP, FGF8, and the homeodomain protein soulless/Phox2a
Development of noradrenergic neurons in the zebrafish hindbrain requires BMP, FGF8, and the homeodomain protein soulless/Phox2a
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DOI：
10.1016/s0896-6273(00)81112-5
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发表时间：
1999-11-01
期刊：
影响因子：
16.2
通讯作者：
Rosenthal, A
中科院分区：
文献类型：
--
作者：
Guo, S;Brush, J;Rosenthal, A
我们报道了斑马鱼的无灵魂突变，其中蓝斑（LC）去甲肾上腺素（NA）神经元未能发育，破坏了同源域蛋白Phox2a。 Phox2a 不仅是必要的，而且足以在异位位置诱导 Phox2b(+) 多巴胺-β-羟化酶(+) 和酪氨酸羟化酶(+) NA 神经元。 Phox2a 首先在背侧前后脑的 LC 祖细胞中检测到，其表达依赖于中脑/后脑边界的 FGF8 以及来自表皮外胚层/未来背侧神经板连接处的 BMP 信号的最佳浓度。这些发现表明，Phox2a 部分通过 Phox2b 的诱导以及响应沿神经板的中外侧和前后轴作用的协作信号来协调 LC 的规范。
We report that the zebrafish mutation soulless, in which the development of locus coeruleus (LC) noradrenergic (NA) neurons failed to occur, disrupts the homeodomain protein Phox2a. Phox2a is not only necessary but also sufficient to induce Phox2b(+) dopamine-beta-hydroxylase(+) and tyrosine hydroxylase(+) NA neurons in ectopic locations. Phox2a is first detected in LC progenitors in the dorsal anterior hindbrain, and its expression there is dependent on FGF8 from the mid/hindbrain boundary and on optimal concentrations of BMP signal from the epidermal ectoderm/future dorsal neural plate junction. These findings suggest that Phox2a coordinates the specification of LC in part through the induction of Phox2b and in response to cooperating signals that operate along the mediolateral and anteroposterior axes of the neural plate.