Molecular characterisation of two paralogous SPO11 homologues in Arabidopsis thaliana
Molecular characterisation of two paralogous SPO11 homologues in Arabidopsis thaliana
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DOI：
10.1093/nar/28.7.1548
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发表时间：
2000-04-01
影响因子：
14.9
通讯作者：
Puchta, H
中科院分区：
文献类型：
--
作者：
Hartung, F;Puchta, H
已发现酵母的Spo 11蛋白与减数分裂中的双链断裂共价结合，表明该蛋白在这些断裂的形成中具有独特的作用。SPO 11基因的同源物已在各种真核生物中发现，表明减数分裂重组中涉及的机制在真核生物中是保守的。在这里，我们报告SPO 11同源植物。与已知的其他真核生物相比，拟南芥基因组中携带至少两个SPO 11同源物AtSPO 11 -1和AtSPO 11 -2，这两个基因彼此之间的关系并不比与其他真核生物SPO 11同源物的关系更密切，表明它们不是在高等植物进化过程中通过最近的复制事件产生的。AtSPO 11 -1不仅在生殖器官中表达，而且在体细胞组织中也有少量表达。我们能够在不同的器官中检测到不同的AtSPO 11 -1 cDNA，其中内含子的剪接方式不同，这是一个令人惊讶的现象，也报告了在哺乳动物中的SPO 11同源物。在AtSPO 11 -2的情况下，我们发现mRNA的3'末端与位于其旁边的反向基因产生的mRNA重叠，这指出了一种可能的反义调控机制。我们的研究结果暗示了一种有趣的可能性，即在植物的盛宴上，Spell样蛋白可能比最初预期的酵母具有更多甚至可能是其他的生物功能。
The Spo11 protein of yeast has been found to be covalently bound to double-strand breaks in meiosis, demonstrating a unique role of the protein in the formation of these breaks. Homologues of the SPO11 gene have been found in various eukaryotes, indicating that the machinery involved in meiotic recombination is conserved in eukaryotes. Here we report on SPO11 homologues in plants. in contrast to what is known from other eukaryotes, Arabidopsis thaliana carries in its genome at least two SPO11 homologues, AtSPO11-1 and AtSPO11-2, Both genes are not more closely related to each other than to other eukaryotic SPO11 homologues, indicating that they did not arise via a recent duplication event during higher plant evolution. For both genes three different polyadenylation sites were found. AtSPO11-1 is expressed not only in generative but also to a lesser extent in somatic tissues. We were able to detect in different organs various AtSPO11-1 cDNAs in which introns were differently spliced-a surprising phenomenon also reported for SPO11 homologues in mammals. In the case of AtSPO11-2 we found that the 3' end of the mRNA is overlapping with a mRNA produced by a gene located in inverse orientation next to it. This points to a possible antisense regulation mechanism. Our findings hint to the intriguing possibility that, at feast for plants, Spell-like proteins might have more and possibly other biological functions than originally anticipated for yeast.