Growth Factors, CD34 Positive Cells, and Fibrin Network Analysis in Concentrated Growth Factors Fraction
Growth Factors, CD34 Positive Cells, and Fibrin Network Analysis in Concentrated Growth Factors Fraction
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DOI：
10.1002/jemt.20968
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发表时间：
2011-08-01
影响因子：
2.5
通讯作者：
Rezzani, Rita
中科院分区：
文献类型：
--
作者：
Rodella, Luigi Fabrizio;Favero, Gaia;Rezzani, Rita
优化愈合组织的一个有趣的临床选择是使用血小板浓缩物。血小板含有大量的生长因子，其中包括已知与组织再生有关的TGF-β 1和VEGF。CGF是通过用特殊的离心装置处理血液样品产生的;形成三层：顶部无细胞血浆（PPP），中间CGF和底部红细胞（RBC）层。鉴于迄今为止还没有关于CGF生物学特性的数据，本研究的目的是评价CGF以及PPP和RBC层中TGF-β 1和VEGF的存在。此外，由于循环干细胞从血液中募集到受伤组织中用于愈合，我们还评估了CD 34阳性细胞的存在。我们的数据显示CGF和RBC层中存在TGF-β 1和VEGF。此外，我们在CGF中显示了CD 34阳性细胞。Microsc. Res. Tech. 74：772-777，2011. (C)2010 Wiley-Liss，Inc.
An interesting clinical option for optimizing healing tissue is the use of platelet concentrate. Platelets contain high quantities of growth factors, among these TGF-beta 1 and VEGF, which are known to be implicated in tissue regeneration. CGF is produced by processing blood samples with a special centrifuge device; three layers are formed: top acellular plasma (PPP), middle CGF and bottom red blood cells (RBC) layers. Given that to date there are no data concerning the biological characteristic of CGF, the aim of this study was to evaluate the presence of TGF-beta 1 and VEGF in CGF and also in PPP and RBC layers. In addition, since circulating stem cells are recruited from blood to injured tissue for healing we also evaluated the presence of CD34 positive cells. Our data show the presence of TGF-beta 1 and VEGF in CGF and RBC layers. In addition, we show CD34 positive cells in CGF. Microsc. Res. Tech. 74:772-777, 2011. (C) 2010 Wiley-Liss, Inc.