Adenovirus-mediated gene therapy of gastric carcinoma using cancer-specific gene expression in vivo.
Adenovirus-mediated gene therapy of gastric carcinoma using cancer-specific gene expression in vivo.
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利用体内癌症特异性基因表达进行腺病毒介导的胃癌基因治疗。
DOI：
10.1006/bbrc.1997.6124
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发表时间：
1997
影响因子：
3.1
通讯作者：
Masao Omata
中科院分区：
文献类型：
--
作者：
Torao Tanaka;F. Kanai;K. Lan;Makoto Ohashi;Y. Shiratori;Yoko Yoshida;Hirofumi Hamada;Masao Omata
癌胚抗原（CEA）是一种糖蛋白，在大多数人胃癌中过表达。我们证明，重组腺病毒载体（AdCEAtk），含有CEA启动子，可以转移单纯疱疹病毒胸苷激酶（HSVtk）基因到CEA生产的胃癌细胞，赋予更昔洛韦（GCV）在体内的敏感性。在离体实验中，当肿瘤含有超过20%的AdCEAtk感染的细胞时，GCV处理后肿瘤生长受到抑制，表明有效的旁观者杀伤效应。肿瘤内注射AdCEAtk后，HSVtk在约30%的产生CEA的癌细胞中选择性表达。通过AdCEAtk注射和GCV施用，与未处理的肿瘤相比，肿瘤的生长被显著抑制20%。本研究为CEA阳性胃癌的肿瘤特异性基因转移提供了一种新的策略。
The carcinoembryonic antigen (CEA) is a glycoprotein which overexpressed in the majority of human gastric cancers. We demonstrated that recombinant adenoviral vector (AdCEAtk), containing the CEA promoter, could transfer the herpes simplex virus thymidine kinase (HSVtk) gene into CEA-producing gastric cancer cells to confer sensitivity to ganciclovir (GCV) in vivo. In an ex vivo experiment, the tumor growth was inhibited after GCV treatment when the tumor contained more than 20% of AdCEAtk infected cells, indicating an efficient bystander killing effect. With intra-tumoral injection of AdCEAtk, the HSVtk were selectively expressed in approximately 30% of CEA producing cancer cells. By AdCEAtk injection and GCV administration, the growth of tumors was significantly inhibited by 20% as compared to untreated tumors. It is hoped that these results provide a strategy of tumor specific gene transfer for CEA producing gastric cancers.
体内肝脏基因治疗：彻底但短暂地纠正 B 型血友病犬的 IX 因子缺乏症。
DOI：
10.1073/pnas.91.6.2353
发表时间：
1994
影响因子：
11.1
作者：
Kay,MA;Landen,CN;Rothenberg,SR;Taylor,LA;Leland,F;Wiehle,S;Fang,B;Bellinger,D;Finegold,M;Thompson,AR
通讯作者：
Thompson,AR