Nanoroughened plasmonic films for enhanced biosensing detection
Nanoroughened plasmonic films for enhanced biosensing detection
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DOI：
10.1088/0957-4484/20/22/225502
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发表时间：
2009-06
期刊：
影响因子：
3.5
通讯作者：
E. Le Moal;S. Lévêque-Fort;M. Potier;E. Fort
中科院分区：
文献类型：
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作者：
E. Le Moal;S. Lévêque-Fort;M. Potier;E. Fort
虽然荧光是生物传感应用中的主流标记技术，但灵敏度的提高仍然是一个艰巨的挑战。我们表明，涂层标准显微镜载玻片与纳米粗糙银膜提供了一个高的荧光信号增强由于等离子体相互作用。作为概念的证明，我们将这些具有定制等离子体特性的薄膜应用于DNA微阵列。使用普通的光学扫描设备，我们实现了超过40倍的信号放大。
Although fluorescence is the prevailing labeling technique in biosensing applications, sensitivity improvement is still a striving challenge. We show that coating standard microscope slides with nanoroughened silver films provides a high fluorescence signal enhancement due to plasmonic interactions. As a proof of concept, we applied these films with tailored plasmonic properties to DNA microarrays. Using common optical scanning devices, we achieved signal amplifications of more than 40-fold.