OXYGEN-AFFINITY AS AN INDEX OF HEMOGLOBIN S POLYMERIZATION - NEW MICROMETHOD
OXYGEN-AFFINITY AS AN INDEX OF HEMOGLOBIN S POLYMERIZATION - NEW MICROMETHOD
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DOI：
10.1016/0003-2697(78)90737-6
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
BENESCH, R
中科院分区：
文献类型：
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作者：
BENESCH, RE;EDALJI, R;BENESCH, R
O2 亲和力随着[人] Hb 浓度的增加而降低（发生在纯 Hb S 溶液中），可用于确定发生脱氧形式聚合的最低浓度。在此基础上开发了一种非常灵敏的测量最小胶凝浓度的方法。描述了温度、pH 和其他 Hb 对通过该方法获得的终点的影响。在所有情况下，观察到与直接在较大样品上确定的最小胶凝浓度非常一致。随着红细胞内 Hb 浓度的增加，含有 Hb S 的红细胞的 O2 亲和力降低，这显然是 Hb S 单独的胶凝特性的直接结果。
The decrease in O2 affinity with increasing [human] Hb concentration, which occurs in solutions of pure Hb S, can be used to determine the minimum concentration at which polymerization of the deoxy form takes place. On this basis a very sensitive method for measuring the minimum gelling concentration was developed. The influence of temperature, pH and other Hb on the end points obtained by this method was described. In all cases excellent aggreement with the minimum gelling concentration determined directly on larger samples was observed. The decrease in O2 affinity of red cells containing Hb S with increasing intracorpuscular Hb concentration is apparently a direct consequence of the gelling properties of Hb S alone.