The Adjuvant Activity of Epimedium Polysaccharide-Propolis Flavone Liposome on Enhancing Immune Responses to Inactivated Porcine Circovirus Vaccine in Mice.
The Adjuvant Activity of Epimedium Polysaccharide-Propolis Flavone Liposome on Enhancing Immune Responses to Inactivated Porcine Circovirus Vaccine in Mice.
复制标题
淫羊藿多糖-蜂胶黄酮脂质体增强猪圆环病毒灭活疫苗小鼠免疫反应的佐剂活性
DOI：
10.1155/2015/972083
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Fan Y;Guo L;Hou W;Guo C;Zhang W;Ma X;Ma L;Song X
目标。研究了淫羊藿多糖-蜂胶黄酮脂质体（EPL）的体外和体内佐剂活性。方法。体外观察不同浓度EPL对大鼠脾淋巴细胞增殖及IFN-γ、IL-6 mRNA表达的影响。在体内，通过接种猪圆环病毒2型（PCV2）灭活疫苗，比较EPL、EP和矿物油对BALB/c小鼠的佐剂活性。结果。EPL在体外促进淋巴细胞增殖，提高IFN-γ和IL-6 mRNA表达，且各浓度均显著优于EP。在体内，EPL显著促进淋巴细胞增殖和细胞因子分泌，提高NK细胞杀伤活性、pcv2特异性抗体滴度和t细胞亚群比例。在大多数时间点，EPL的效果明显优于EP和油佐剂。结论。EPL能显著提高pcv2特异性细胞免疫应答和体液免疫应答，以中剂量效果最好。因此，EPL可作为PCV2灭活疫苗的有效免疫佐剂。
Objectives. The adjuvant activity of Epimedium polysaccharide-propolis flavone liposome (EPL) was investigated in vitro and in vivo. Methods. In vitro, the effects of EPL at different concentrations on splenic lymphocytes proliferation and mRNA expression of IFN-γ and IL-6 were determined. In vivo, the adjuvant activities of EPL, EP, and mineral oil were compared in BALB/c mice through vaccination with inactivated porcine circovirus type 2 (PCV2) vaccine. Results. In vitro, EPL promoted lymphocytes proliferation and increased the mRNA expression of IFN-γ and IL-6, and the effect was significantly better than EP at all concentrations. In vivo, EPL significantly promoted the lymphocytes proliferation and the secretion of cytokines and improved the killing activity of NK cells, PCV2-specific antibody titers, and the proportion of T-cell subgroups. The effects of EPL were significantly better than EP and oil adjuvant at most time points. Conclusion. EPL could significantly improve both PCV2-specific cellular and humoral immune responses, and its medium dose had the best efficacy. Therefore, EPL would be exploited in an effective immune adjuvant for inactivated PCV2 vaccine.