Lineage Tracing
Lineage Tracing
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DOI：
10.1016/j.cell.2012.01.002
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发表时间：
2012-01-20
期刊：
影响因子：
64.5
通讯作者：
Watt, Fiona M.
中科院分区：
文献类型：
--
作者：
Kretzschmar, Kai;Watt, Fiona M.
谱系追踪是鉴定单个细胞的所有后代。虽然它的起源可以追溯到世纪无脊椎动物的发育生物学，谱系追踪现在是研究成年哺乳动物组织中干细胞特性的重要工具。谱系追踪为理解组织发育、稳态和疾病提供了强有力的手段，特别是当它与调节细胞命运决定的信号的实验操作相结合时。最近，诱导型重组酶，荧光报告构建体和活细胞成像的组合为干细胞生物学提供了前所未有的见解。在这里，我们讨论了不同的实验策略，目前可用于谱系追踪，其相关的警告，和新的机会，整合谱系追踪与细胞内信号通路的监测。
Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19th century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and disease, especially when it is combined with experimental manipulation of signals regulating cell-fate decisions. Recently, the combination of inducible recombinases, multicolor reporter constructs, and live-cell imaging has provided unprecedented insights into stem cell biology. Here we discuss the different experimental strategies currently available for lineage tracing, their associated caveats, and new opportunities to integrate lineage tracing with the monitoring of intracellular signaling pathways.