Models of cytoskeletal mechanics of adherent cells
Models of cytoskeletal mechanics of adherent cells
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DOI：
10.1007/s10237-002-0009-9
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发表时间：
2002-06-01
影响因子：
3.5
通讯作者：
Ingber, D. E.
中科院分区：
文献类型：
--
作者：
Stamenovic, D.;Ingber, D. E.
贴壁细胞感知其机械环境，进而调节其功能。在过去的十年中，越来越多的证据表明，一种可变形的固态细胞内结构，称为细胞骨架（CSK），在细胞内传递和分布机械应力以及将其转化为化学反应方面发挥着重要作用。因此，为了了解细胞功能的机械调节和控制，我们需要了解决定 CSK 如何改变其形状和力学以响应压力的机制。在本次调查中，我们研究了常用的基于结构的 CSK 模型。我们特别关注其中的两类模型：开孔泡沫网络和应力支撑结构。我们确定了决定这些模型可变形性的潜在机制，并将模型预测与之前从稳态培养的活贴壁细胞的机械测试中获得的数据进行比较。我们得出的结论是，应力支撑结构似乎更适合描述细胞变形性，因为这类结构可以解释细胞骨架收缩预应力在细胞力学中发挥的核心作用。
Adherent cells sense their mechanical environment, which, in turn, regulates their functions. During the past decade, a growing body of evidence has indicated that a deformable, solid-state intracellular structure known as the cytoskeleton (CSK) plays a major role in transmitting and distributing mechanical stresses within the cell as well as in their conversion into a chemical response. Therefore in order to understand mechanical regulation and control of cellular functions, one needs to understand mechanisms that determine how the CSK changes its shape and mechanics in response to stress. In this survey, we examined commonly used structurally based models of the CSK. In particular, we focused on two classes of these models: open-cell foam networks and stress-supported structures. We identified the underlying mechanisms that determine deformability of those models and compare model predictions with data previously obtained from mechanical tests on cultured living adherent cells at steady state. We concluded that stress-supported structures appear more suitable for describing cell deformability because this class of structures can explain the central role that the cytoskeletal contractile prestress plays in cellular mechanics.