Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina
Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina
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红树林白骨壤3个CBF/DREB1转录因子基因的鉴定及表达分析
DOI：
10.1016/j.aquatox.2013.05.014
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发表时间：
2013-09-15
影响因子：
4.5
通讯作者：
Fei, Jiao
中科院分区：
文献类型：
--
作者：
Peng, Ya-Lan;Wang, You-Shao;Fei, Jiao
从白骨壤中分离到3个CBF/DREB1(C-重复结合因子/脱水反应元件结合因子1)同源物，分别命名为AmCBF1、2和3。多重序列分析表明，这3个蛋白都含有一个AP2 DNA结合域和两个CBF特征序列。根据系统发育分析，这些蛋白属于DREB亚家族的A-1亚群。基于实时荧光定量聚合酶链式反应的表达分析表明，AmCBF2在正常条件下表现出较高的表达，在茎中的表达水平最高，而AmCBF1和AmCBF3在没有胁迫的情况下都弱表达。这三个基因对不同的环境刺激表现出不同的反应。AmCBF2受低温、干旱、高盐度、重金属和脱落酸(ABA)的诱导，且受寒冷、干旱、Pb2+和Zn2+的诱导比受NaC l、ABA和Cd 2+的诱导强得多。相反，AmCBF1和AmCBF3在这些刺激下的变化都不显著。这些结果表明，这三个AmCBF基因在滨海对虾中发挥着不同的作用，AmCBF2基因可能参与了寒冷、干旱和重金属胁迫反应的信号通路。(C)2013爱思唯尔B.V.保留所有权利。
Three CBF/DREB1 (C-repeat binding factor/dehydration responsive element-binding factor 1) homologues were isolated from mangrove Avicennia marina and designated AmCBF1, 2 and 3. Multiple sequence analysis showed that the three deduced proteins all contain an AP2 DNA-binding domain and two CBF signature sequences. According to the phylogenetic analysis, these proteins belong to the A-1 subgroup of the DREB subfamily. Expression analyses based on quantitative real-time PCR revealed that the AmCBF2 displayed relatively high expression under normal conditions, with the highest level in stems, while both AmCBF1 and 3 were weakly expressed without stress. The three genes also showed different responses to various environmental stimuli. The AmCBF2 was inducible by cold, drought, high salinity, heavy metals, as well as abscisic acid (ABA), and exhibited much stronger induction by cold, drought, Pb2+ or Zn2+ than by NaCl, ABA or Cd2+. In contrast, both AmCBF1 and AmCBF3 displayed insignificant changes under these stimuli. These results indicate that the three AmCBF genes play different roles in A. marina and the AmCBF2 might be involved in the signaling pathway of cold, drought and heavy metal stress response. (C) 2013 Elsevier B.V. All rights reserved.