Release of nitric oxide together with carbon-centered radicals from N-nitrosamines by ultraviolet light irradiation
Release of nitric oxide together with carbon-centered radicals from N-nitrosamines by ultraviolet light irradiation
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DOI：
10.1080/10715760100301301
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发表时间：
2001-01-01
影响因子：
3.3
通讯作者：
Kikugawa, K
中科院分区：
文献类型：
--
作者：
Hiramoto, K;Ohkawa, T;Kikugawa, K
在室温下，用紫外光照射N-亚硝胺、N-亚硝基二甲胺、N-亚硝基二乙胺、N-亚硝基吗啉和N-亚硝基吡咯烷在磷酸盐缓冲液(pH 7.4)中的溶液。紫外吸收或高效液相分析表明，N-亚硝胺在辐照120min后被大量降解，且降解呈时间依赖关系。用5，5-二甲基-1-吡咯啉-N-氧化物和N-叔丁基-α-苯基硝酮作为自旋陷阱，用电子自旋共振技术监测到，在10-60 S的短时辐照过程中，4个N-亚硝胺产生了碳中心自由基。用半胱氨酸铁(II)络合物、N-甲基-D-氨基葡萄糖二硫代氨基甲酸酯和2-(4-carboxyphenyl)-4，4，5，5-tetramethylimidazoline-1-oxyl-3-oxide.的电子自旋共振技术监测到，在短时照射过程中产生了一氧化氮(NO在120min的照射时间内，4种N-亚硝胺产生大量亚硝酸盐(4-16%)，N-亚硝基二甲胺产生大量硝酸盐(4%)。从N-亚硝胺中释放的NO必须通过中间活性氮氧化物物种，包括二氧化氮和三氧化二氮与溶解氧接触而转化为亚硝酸盐。
Solutions of N-nitrosamines, N-nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosomorpholine and N-nitrosopyrrolidine in phosphate buffer (pH 7.4) were irradiated by ultraviolet (UV) light at room temperature. The N-nitrosamines were extensively degraded due to irradiation for 120min in a time-dependent fashion as monitored by UV-absorption or high performance liquid chromatographic analysis. Carbon-centered radicals were generated from four N-nitrosamines during the short time irradiation of 10-60 s as monitored by electron spin resonance (ESR) technique using 5,5-dimethyl-1-pyrroline N-oxide and N-tert-butyl-alpha-phenylnitrone as spin traps. Nitric oxide (NO) was generated during the short time irradiation as monitored by ESR technique using cysteine-Fe(II) complex, N-methyl-D-glucamine dithdocarbamate and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. Significant amounts of nitrite (4-16%) from four N-nitrosamines and also a significant amount of nitrate (4%) was produced from N-nitrosodimethylamine during the irradiation time of 120 min. Released NO from the N-nitrosamines must be converted into nitrite through intermediary reactive nitrogen oxide species including nitrogen dioxide and dinitrogen trioxide in contact with dissolved oxygen.