Role of moving growth cone-like "wave" structures in the outgrowth of cultured hippocampal axons and dendrites
Role of moving growth cone-like "wave" structures in the outgrowth of cultured hippocampal axons and dendrites
复制标题
DOI：
10.1002/(sici)1097-4695(199904)39:1
复制
发表时间：
1999-04-01
期刊：
影响因子：
--
通讯作者：
Banker, G
中科院分区：
文献类型：
--
作者：
Ruthel, G;Banker, G
海马体神经元表现出周期性的生长锥形结构，称为“波”，从神经突的底部出现，并向远端传播到尖端。当波靠近尖端时，神经突会收缩，当波到达尖端时，神经突会迅速生长。在培养的第1天，在轴突生长的早期，轴突在波到达尖端之前平均收缩7.5 μ m，然后在波到达尖端后立即生长12 μ m（平均净生长4.5 μ m）。在分叉的轴突中，波通常有选择地沿着一个分支或另一个分支传播，生长选择性地发生在波所选择的分支上，在平均生长速度要慢得多的树突中，波相关的收缩要大得多，导致净生长较少。Brefeldin A的存在会破坏通过高尔基体的膜交通并导致轴突的收缩，轴突波继续与生长突和收缩有关。与未处理的轴突相比，生长突的幅度没有显著差异，但与波相关的收缩明显增加。波与周期性伸长之间的密切联系表明，波可能会导致这种生长模式。我们的研究结果还表明，规律发生的缩回相的调制在决定平均生长速率方面起着突出作用。(C) 1999 John Wiley & Sons, Inc
Hippocampal neurons exhibit periodically recurring growth cone-like structures, referred to as "waves," that emerge at the base of neurites and travel distally to the tip. As a wave nears the tip, the neurite undergoes retraction, and when it reaches the tip, the neurite undergoes a burst of growth. At 1 day in culture, during early axon outgrowth, axone undergo an average 7,5-mu m retraction immediately preceding wave arrival at the tip followed by 12-mu m growth immediately after arrival tan average net growth of 4.5 mu m). In branched axons, waves often selectively travel down one branch or the other, Growth selectively occurs in the branch chosen by the wave, In dendrites, which grow much slower on average, wave-associated retractions are much greater, resulting in less net growth. In the presence of Brefeldin A, which disrupts membrane traffic through the Golgi apparatus and leads to retraction of the axon, axonal waves continue to be associated with both growth spurts and retractions. The magnitude of the growth spurts is not significantly different from untreated axons, but wave-associated retractions are significantly increased, The close association between waves and cyclical elongation suggests that waves may act to bring about this pattern of growth. Our results also show that modulation of regularly occurring retraction phases plays a prominent role in determining average outgrowth rates. (C) 1999 John Wiley & Sons, Inc.