Evaluation of Papanicolaou stain for studying micronuclei in buccal cells under field conditions.
Evaluation of Papanicolaou stain for studying micronuclei in buccal cells under field conditions.
复制标题
评估巴氏染色在现场条件下研究口腔细胞微核的效果。
DOI：
10.1159/000325980
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发表时间：
2006
期刊：
影响因子：
1.8
通讯作者：
Loffredo,ChristopherA
中科院分区：
文献类型：
--
作者：
Ayyad,SohairBA;Israel,Ebenezer;El-Setouhy,Maged;Nasr,GhadaRadwan;Mohamed,MostafaK;Loffredo,ChristopherA
ObjectiveTo compare Papanicolaou (Pap) and May-Grünwald Giemsa (MGG) stain as 2 techniques for staining for buccal mucosal cells to detect micronuclei (MN) infield studies.Study designEighty cytologic smears (2 per individual) were taken from the buccal mucosa of 40 cigarette smokers recruited at a rural village in Egypt. Forty smears were stained with Pap stain and 40 with MGG stain. All were assessed for cellularity and scored for MN.ResultsPap stain was faster and easier to process and transport in the field study than was MGG stain. Regarding MGG smears, bacteria and cell debris masked the MN as compared to Pap smears, in which the fixative destroyed the bacteria and made the cell boundaries clearly demarcated. Using Pap stain, MN were seen easily in transparent cytoplasm.ConclusionPap stain is the preferred method infield studies for scoring and detecting MN in cells of buccal mucosa.
