5-COMPONENT DIELECTRIC-DISPERSION IN BOVINE SERUM-ALBUMIN SOLUTION
5-COMPONENT DIELECTRIC-DISPERSION IN BOVINE SERUM-ALBUMIN SOLUTION
复制标题
DOI：
10.1088/0031-9155/22/6/008
复制
发表时间：
1977-01-01
影响因子：
3.5
通讯作者：
PEETERS, H
中科院分区：
文献类型：
--
作者：
ESSEX, CG;SYMONDS, MS;PEETERS, H
在 3 个浓度和 0.03-800 MHz 范围内的约 80 个独立频率下对天然 BSA（牛血清白蛋白）单体的水溶液进行介电测量。所有测量均在 25°C 下进行。 C 溶液的 pH = 5.0，接近等电点。分析表明存在双组分β。频率低于 10 MHz 加 2 δ 时的色散。弛豫频率约为 15 MHz 和 100 MHz 的色散。 β分子水平的起源。以及更高的频率.delta。可以放心地确定分散度，但剩余的 delta。色散不能被明确地解释。随着众所周知的.gamma 的加入。在频率超过 1 GHz 时的色散，测量结果表明存在 5 个明显分离的色散区域。这是比之前文献中建议的这种性质的蛋白质溶液更多的分散体。
Dielectric measurements were made on aqueous solutions of native BSA [bovine serum albumin] monomers at 3 concentrations and at about 80 separate frequencies in the range 0.03-800 MHz. All measurements were made at 25.degree. C on solutions of pH = 5.0, this being near the isoelectric point. The analysis shows the presence of a double component .beta. dispersion at frequencies below 10 MHz plus 2 .delta. dispersions with relaxation frequencies of around 15 MHz and 100 MHz. The origin at a molecular level of the .beta. and the higher frequency .delta. dispersion can be established with confidence but the remaining .delta. dispersion cannot be interpreted unambiguously. With the inclusion of the well known .gamma. dispersion at frequencies in excess of 1 GHz the measurements indicate the existence of 5 clearly separated dispersion regions. This is one more dispersion than previously suggested in the literature for a protein solution of this nature.