Fabrication of ZnPc/protein nanohorns for double photodynamic and hyperthermic cancer phototherapy
Fabrication of ZnPc/protein nanohorns for double photodynamic and hyperthermic cancer phototherapy
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DOI：
10.1073/pnas.0801349105
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发表时间：
2008-09-30
影响因子：
11.1
通讯作者：
Yudasaka, Masako
中科院分区：
文献类型：
--
作者：
Zhang, Minfang;Murakami, Tatsuya;Yudasaka, Masako
通过附着功能分子可以轻松实现碳纳米管的多功能化，从而为微观应用提供特定的优势。我们制造了使用单激光的双重光动力疗法（PDT）和光热疗法（PHT）癌症光疗系统。将锌酞菁（ZnPc）负载到开孔的单壁碳纳米角（SWNHox）上，并将牛血清白蛋白（BSA）连接到SWNHox的羧基上。在该系统中，ZnPc是PDT剂，SWNHox是PHT剂，BSA增强了生物相容性。双重光疗效果在体外和体内得到证实。当将ZnPc-SWNHox-BSA注射到皮下移植到小鼠体内的肿瘤中时，在670 nm激光照射下，肿瘤几乎消失。相反，当仅注射 ZnPc 或 SWNHox-BSA 时，肿瘤继续生长。我们的结论是，碳纳米管可能是用于癌症光疗的有价值的新工具。
Multifunctionalization of carbon nanotubules is easily achieved by attaching functional molecules that provide specific advantages for microscopic applications. We fabricated a double photodynamic therapy (PDT) and photohyperthermia (PHT) cancer phototherapy system that uses a single laser. Zinc phthalocyanine (ZnPc) was loaded onto single-wall carbon nanohorns with holes opened (SWNHox), and the protein bovine serum albumin (BSA) was attached to the carboxyl groups of SWNHox. In this system, ZnPc was the PDT agent, SWNHox was the PHT agent, and BSA enhanced biocompatibility. The double phototherapy effect was confirmed in vitro and in vivo. When ZnPc-SWNHox-BSA was injected into tumors that were subcutaneously transplanted into mice, the tumors almost disappeared upon 670-nm laser irradiation. In contrast, the tumors continued to grow when only ZnPc or SWNHox-BSA was injected. We conclude that carbon nanotubules may be a valuable new tool for use in cancer phototherapy.