Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
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DOI：
10.1261/rna.5235104
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发表时间：
2004-03-01
期刊：
影响因子：
4.5
通讯作者：
Tuschl, T
中科院分区：
文献类型：
--
作者：
Meister, G;Landthaler, M;Tuschl, T
近年来，在植物和动物中发现了大量的mirna。抑制microRNA功能的反向遗传方法的发展将促进这类新的非编码RNA的研究。在这里，我们发现2'- o -甲基寡核苷酸，而不是2'-脱氧寡核苷酸特异性地灭活与mirna -蛋白复合物相关的RNAi活性，在人类细胞提取物和培养的人类细胞中。
A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells.