[Effect of glucocorticoid on [Ca2+]i induced by high-K+ in single PC12 cells and its characteristics].
[Effect of glucocorticoid on [Ca2+]i induced by high-K+ in single PC12 cells and its characteristics].
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发表时间：
1998-10
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通讯作者：
S. Lou;C. Wang;X. Huang;Y. Chen
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作者：
S. Lou;C. Wang;X. Huang;Y. Chen
在嗜铬细胞瘤 PC12 细胞中研究了糖皮质激素对高 K+ 诱导的胞质游离钙 [Ca2+]i) 增加的影响。主要结果如下：（1）当细胞在10（-5）mol/L皮质酮存在下于37℃预孵育不同时间间隔并用55mmol/L KCl刺激时，观察到皮质酮对δ[Ca2+]i的抑制作用呈时间依赖性，第5分钟作用最大，第25分钟没有作用。 (2)PC12细胞与不同浓度的皮质酮在37℃下预孵育5 min时，皮质酮对55 mmol/L KCl诱导的δ[Ca2+]i的抑制作用呈浓度依赖性。发现该作用在皮质酮10(-5)mol/L时最大，在皮质酮10(-9)mol/L时消失。 (3)其他类固醇如皮质醇、地塞米松、黄体酮、睾酮、17β-雌二醇、醛固酮等也不同程度地抑制PC12细胞中55 mmol/L KCl引起的[Ca2+]i升高，即P=F>Dex>T>Ald=E2。胆固醇在浓度高达10(-5) mol/L时对55 mmol/L KCl引起的[Ca2+]i抑制无效。 (4)皮质酮不能抑制细胞外钙浓度从无Ca(2+)到3 mmol/L引起的[Ca2+]i升高。
The effect of glucocorticoid on the increase of cytosolic free calcium [Ca2+]i) induced by high-K+ was investigated in pheochomocytoma PC12 cells. The main result was as follows: (1) When the cells were preincubated at 37 degrees C for various time intervals in the presence of 10(-5) mol/L of corticosterone and stimulated with 55 mmol/L KCl, an inhibitory effect of corticosterone on delta [Ca2+]i was observed in a time-dependent manner with maximal effect at 5th min and no effect at 25th min. (2) When PC12 cells were preincubated with various concentration of corticosterone at 37 degrees C for 5 min, the inhibitory effect of corticosterone on delta [Ca2+]i induced by 55 mmol/L KCl was concentration-dependent. The effect was found to be maximal at 10(-5) mol/L of corticosterone and disappeared at 10(-9) mol/L of corticosterone. (3) Other steroids such as cortisol, dexamethasone, progesterone, testosterone, 17 beta-estradiol and aldosterone also inhibited the rise of [Ca2+]i evoked by 55 mmol/L KCl in PC12 cells to different extents, i.e., in the rank of P = F > Dex > T > Ald = E2. Cholesterol was ineffective at the concentration up to 10(-5) mol/L for inhibiting [Ca2+]i evoked by 55 mmol/L KCl. (4) Corticosterone could not inhibit the [Ca2+]i rise induced by changing the concentration of extracellular calcium from Ca(2+)-free to 3 mmol/L.