Inhibitory Gating of Basolateral Amygdala Inputs to the Prefrontal Cortex
Inhibitory Gating of Basolateral Amygdala Inputs to the Prefrontal Cortex
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DOI：
10.1523/jneurosci.0874-16.2016
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发表时间：
2016-09-07
影响因子：
5.3
通讯作者：
Carter, Adam G.
中科院分区：
文献类型：
--
作者：
McGarry, Laura M.;Carter, Adam G.
前额叶皮层（PFC）和基底外侧杏仁核（BLA）之间的相互作用调节情绪行为。然而，对这些大脑区域之间的功能连接的回路水平的理解仍然不完整。BLA向PFC发送突出的神经元投射，但这些输入的总体影响主要是抑制性的。在这里，我们结合联合收割机有针对性的记录和光遗传学检查这种抑制在小鼠边缘下PFC的突触基础。我们发现，BLA输入优先目标层2皮质杏仁核在相邻的皮质纹状体神经元。然而，这些输入与邻近的小白蛋白和生长抑素表达中间神经元建立了更强的连接。来自这两个中间神经元群体的抑制性连接在皮质杏仁核神经元上也强得多。因此，BLA输入能够通过两个平行的中间神经元通路驱动鲁棒的前馈抑制。此外，由于短期突触动力学的差异，这些中间神经元的贡献在重复活动期间发生变化。因此，小清蛋白中间神经元在刺激序列开始时被激活，而生长抑素中间神经元激活在这些序列期间建立。总之，这些结果揭示了BLA如何通过直接兴奋和前馈抑制的复杂相互作用影响PFC。他们还强调了在这个皮层回路中多个投射神经元和中间神经元的靶向连接的作用。我们的研究结果为BLA如何影响PFC电路提供了一个机械的理解，对该电路如何参与情绪调节具有重要意义。
Interactions between the prefrontal cortex (PFC) and basolateral amygdala (BLA) regulate emotional behaviors. However, a circuit-level understanding of functional connections between these brain regions remains incomplete. The BLA sends prominent glutamatergic projections to the PFC, but the overall influence of these inputs is predominantly inhibitory. Here we combine targeted recordings and optogenetics to examine the synaptic underpinnings of this inhibition in the mouse infralimbic PFC. We find that BLA inputs preferentially target layer 2 corticoamygdala over neighboring corticostriatal neurons. However, these inputs make even stronger connections onto neighboring parvalbumin and somatostatin expressing interneurons. Inhibitory connections from these two populations of interneurons are also much stronger onto corticoamygdala neurons. Consequently, BLA inputs are able to drive robust feedforward inhibition via two parallel interneuron pathways. Moreover, the contributions of these interneurons shift during repetitive activity, due to differences in short-term synaptic dynamics. Thus, parvalbumin interneurons are activated at the start of stimulus trains, whereas somatostatin interneuron activation builds during these trains. Together, these results reveal how the BLA impacts the PFC through a complex interplay of direct excitation and feedforward inhibition. They also highlight the roles of targeted connections onto multiple projection neurons and interneurons in this cortical circuit. Our findings provide a mechanistic understanding for how the BLA can influence the PFC circuit, with important implications for how this circuit participates in the regulation of emotion.