Allyl Isothiocyanate in the Volatiles of Brassica juncea Inhibits the Growth of Root Rot Pathogens of Panax notoginseng by Inducing the Accumulation of ROS
Allyl Isothiocyanate in the Volatiles of Brassica juncea Inhibits the Growth of Root Rot Pathogens of Panax notoginseng by Inducing the Accumulation of ROS
复制标题
芥菜挥发物中异硫氰酸烯丙酯通过诱导ROS积累抑制三七根腐病菌生长
DOI：
10.1021/acs.jafc.1c05225
复制
发表时间：
2021-11-24
影响因子：
6.1
通讯作者：
Zhu, Shusheng
中科院分区：
文献类型：
--
作者：
Liu, Haijiao;Wu, Jiaqing;Zhu, Shusheng
土传病原菌积累引起的根腐病是三七栽培的主要障碍。本文研究了芥菜型油菜挥发物对三七根腐病菌的抑制活性，并对挥发物中的化合物进行了鉴定。此外，通过整合转录组和代谢组分析，阐明了异硫氰酸烯丙酯（AITC）的抗菌活性和机制。B的挥发物。芥菜对根腐病病原体表现出剂量依赖性的抗微生物活性。AITC作为主要的挥发性化合物，不仅在体外显著抑制病原菌的生长，而且在田间也抑制根腐病。综合转录组学和代谢组学分析表明，AITC通过干扰能量产生来抑制茄病镰刀菌，并通过降低谷胱甘肽（GSH）含量来诱导ROS的积累。综上所述，B.芥菜释放AITC抑制土壤传播的病原体，并可用作轮作作物或土壤熏蒸剂，以减轻根腐病。
The cultivation of Panax notoginseng is often seriously hindered by root rot disease caused by the accumulation of soil-borne pathogens. Here, the inhibitory activity of Brassica juncea volatiles on P. notoginseng root rot pathogens was assessed and compounds in volatiles were identified. Furthermore, the antimicrobial activity and mechanism of allyl isothiocyanate (AITC) were deciphered by integrated transcriptome and metabolome analyses. The volatiles of B. juncea showed dose-dependent antimicrobial activity against root rot pathogens. AITC, identified as the main volatile compound, not only significantly inhibited pathogen growth in vitro but also suppressed root rot disease in the field. Integrated transcriptomic and metabolomics analysis revealed that AITC inhibited Fusarium solani by interfering with energy production and induced the accumulation of ROS by decreasing the content of glutathione (GSH). In summary, B. juncea releases AITC to inhibit soil-borne pathogens and could be used as a rotation crop or soil fumigant to alleviate root rot disease.