Effects of sex (or lack thereof) on meibogenesis in mice (Mus musculus): Comparative evaluation of lipidomes and transcriptomes of male and female tarsal plates
Effects of sex (or lack thereof) on meibogenesis in mice (Mus musculus): Comparative evaluation of lipidomes and transcriptomes of male and female tarsal plates
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DOI：
10.1016/j.jtos.2019.03.002
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发表时间：
2019-10-01
期刊：
影响因子：
6.4
通讯作者：
Wilkerson, Amber
中科院分区：
文献类型：
--
作者：
Butovich, Igor A.;McMahon, Anne;Wilkerson, Amber
性别在减数腺脂质生物合成中的可能作用（称为减数发生）仍不清楚。为了确定是否存在任何主要的性别特异性差异在睑板腺分泌物脂质组成（meibum）和基因表达模式（GEP）相关的meibom发生，我们进行了一项研究，使用健康，年龄和饮食匹配的年轻成年野生型C57BL/6J小鼠（2-2.5月龄）。从小鼠眼皮上手术切除跗骨板（TP）并进行转录组学和脂质组学分析。采用mRNA微阵列技术对GEP进行研究。用有机溶剂提取脂质，用液相色谱和质谱法分析。GEP在雌性和雄性小鼠TP中，与脂质代谢和储存相关的主要蛋白编码基因（如Elovl、Scd、Fads、Soat、Far、Awat、Acat、Lss、Dhcr、Hmgcr、Hmgcs、Dgat、Bckdh、Dbt、Fasn、Plin等）的表达水平无统计学差异。雌性和雄性小鼠的肌脂质谱几乎没有区别：所有主要的脂质，如蜡、胆固醇酯、胆固醇、(O)酰化ω -羟基脂肪酸（OAHFA）、OAHFA的胆固醇酯等，都以相似的比例存在。似乎雄性和雌性小鼠的睑板腺的主要生物合成途径以相似的方式发挥作用，产生的分泌物具有相同的总体化学成分。
The possible role of sex in the biosynthesis of lipids in the Meibomian glands (termed meibogenesis) remains unclear. To determine if there were any major sex-specific differences in the lipid composition of meibomian gland secretions (meibum) and gene expression patterns (GEP) related to meibogenesis, we conducted a study using healthy, age and diet-matched young adult wild-type C57BL/6J mice (2-2.5 month old). Tarsal plates (TP) were surgically excised from the eyelids of mice and subjected to transcriptomic and lipidomic analyses. The GEP were studied using mRNA microarrays. Lipids were extracted with organic solvents and analyzed using liquid chromatography and mass spectrometry. GEP in the TP of female and male mice demonstrated no statistically significant differences in the expression levels of the main protein-coding genes related to lipid metabolism and storage in general, and meibogenesis specifically (such as Elovl, Scd, Fads, Soat, Far, Awat, Acat, Lss, Dhcr, Hmgcr, Hmgcs, Dgat, Bckdh, Dbt, Fasn, and Plin, among others). The meibomian lipid profiles of female and male mice were virtually indistinguishable: all major lipids such as waxes, cholesteryl esters, cholesterol, (O)-acylated omega-hydroxy fatty acids (OAHFA), cholesteryl esters of OAHFA etc., were present in similar ratios. It seems that the major biosynthetic pathways in the Meibomian glands of male and female mice function in a similar fashion and produce secretions of the same overall chemical composition.