Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila
Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila
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DOI：
10.1016/j.fsi.2008.05.002
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发表时间：
2008-09-01
影响因子：
4.7
通讯作者：
Figueras, A.
中科院分区：
文献类型：
--
作者：
Rodriguez, I.;Novoa, B.;Figueras, A.
在斑马鱼（Danio rerio）培养设施中分离、鉴定和表征了一株与异常死亡率相关的嗜水气单胞菌。在攻毒实验中，成年斑马鱼易受腹腔注射活菌及其细胞外产物（ECPs）感染，数小时内死亡率极高。由活菌或ECPs引起的感染，由于细菌的细胞毒性和溶血活性，导致肾脏细胞死亡。物。此外，感染影响了活性氧（ROS）和活性氮（NO）自由基的释放。为了确定嗜水单胞杆菌感染是否诱导炎症反应，采用实时PCR技术检测肿瘤坏死因子- α (TNF α)、白细胞介素-1 β (IL-1 β)、干扰素- γ (IFN γ)和诱导型一氧化氮（iNOS） mRNA表达水平。活菌、热灭菌和ECPs感染斑马鱼肾脏后，TNF α、IL-1 β和IFN γ的表达水平上调。ECPs可上调iNOS的表达水平。同时测定死亡率（LD50）和组织病理学。(c) 2008 Elsevier Ltd.版权所有。
A strain of Aeromonas hydrophila associated with unusual mortalities in zebrafish (Danio rerio) culture facilities was isolated, identified and characterized. In challenge experiments, adult zebrafish were susceptible to infection by intraperitoneal (i.p.) injection with viable bacteria and its extracellular products (ECPs) reaching very high mortalities in a few hours. The infection, by the viable bacteria or the ECPs, caused cell death in kidney, due to the cytotoxic and haemolytic activities of the bacterial. ECPs. Moreover, the infection affected the release of oxygen (ROS) and nitrogen (NO) reactive free radicals. To determine if this A. hydrophila infection induces an inflammatory response, mRNA expression levels of tumour necrosis factor-alpha (TNF alpha), interleukin-1 beta (IL-1 beta), interferon-gamma (IFN gamma) and inducible nitric oxide (iNOS) were assessed by real time PCR. The expression levels of TNF alpha, IL-1 beta and IFN gamma were upregulated in the kidneys of infected zebrafish with viable bacteria, heat-killed bacteria and ECPs. Expression levels of iNOS were upregulated by ECPs. Mortality rate (LD50) and histopathology were also determined. (c) 2008 Elsevier Ltd. All rights reserved.