BINDING OF ALPHA-2-MACROGLOBULIN TO HEPATOCYTES - MECHANISM OF INVIVO CLEARANCE
BINDING OF ALPHA-2-MACROGLOBULIN TO HEPATOCYTES - MECHANISM OF INVIVO CLEARANCE
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DOI：
10.1016/0006-291x(85)90117-2
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
PIZZO, SV
中科院分区：
文献类型：
--
作者：
FELDMAN, SR;ROSENBERG, MR;PIZZO, SV
在4 ℃下研究125标记的人α 2-巨球蛋白-甲胺与原代培养的成年大鼠肝细胞的结合。C.培养4小时的细胞在1小时后表现出稳态配体结合，受体数量为22，400个受体/细胞，Kd为0.6 nM。随着培养时间的延长，受体数量明显减少，2天后受体数量仅为初始数量的10%（P < 0.01）。在静脉注射125 I标记的α 2-巨球蛋白-甲胺的小鼠的尸检研究中，在肝脏中发现> 90%的清除的配体。肝脏放射自显影显示80%的配体被肝细胞清除。肝细胞是从受体识别的α 2-巨球蛋白的循环中清除的主要途径。
The binding of 125-labeled human .alpha.2-macroglobulin-methylamine to adult rat hepatocytes in primary culture was studied at 4.degree. C. Cells which were in culture for 4 h exhibited steady-state ligand binding after 1 h, a receptor number of 22,400 receptors/cell, and a Kd of 0.6 nM. Adult rat hepatocytes exhibited a significant decrease in receptor number with increased time in primary culture with < 10% of the initial number of receptors remaining after 2 days (P < 0.01). In autopsy studies of mice injected i.v. with 125I-labeled .alpha.2-macroglobulin-methylamine, > 90% of the cleared ligand was found in the liver. Autoradiography of the liver demonstrated that 80% of the ligand was cleared by hepatocytes. The heptocytes are the primary pathway for clearance from the circulation of receptor recognized .alpha.2-macroglobulin.