Meningococcal Type IV Pili Recruit the Polarity Complex to Cross the Brain Endothelium
Meningococcal Type IV Pili Recruit the Polarity Complex to Cross the Brain Endothelium
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DOI：
10.1126/science.1173196
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发表时间：
2009-07-03
期刊：
影响因子：
56.9
通讯作者：
Nassif, Xavier
中科院分区：
文献类型：
--
作者：
Coureuil, Mathieu;Mikaty, Guillain;Nassif, Xavier
IV型菌毛介导了许多细菌病原体与其宿主细胞的初始相互作用。在脑膜炎奈瑟氏菌中，IV型菌毛介导的细菌需要与脑内皮细胞黏附才能穿过血脑屏障。在这里，IV型菌毛介导的脑膜炎奈瑟菌与人脑内皮细胞的黏附被发现招募了Par3/Par6/PKC Zeta极性复合体，该复合体在真核细胞极性的建立和细胞间连接的形成中起着关键作用。这种招募导致在细菌-宿主细胞相互作用的部位形成异位的细胞间连接结构域，随后随着脑-内皮细胞界面的细胞间连接的开放，细胞-细胞界面的连接蛋白枯竭。
Type IV pili mediate the initial interaction of many bacterial pathogens with their host cells. In Neisseria meningitidis, the causative agent of cerebrospinal meningitis, type IV pili-mediated adhesion to brain endothelial cells is required for bacteria to cross the blood-brain barrier. Here, type IV pili-mediated adhesion of N. meningitidis to human brain endothelial cells was found to recruit the Par3/Par6/PKC zeta polarity complex that plays a pivotal role in the establishment of eukaryotic cell polarity and the formation of intercellular junctions. This recruitment leads to the formation of ectopic intercellular junctional domains at the site of bacteria-host cell interaction and a subsequent depletion of junctional proteins at the cell-cell interface with opening of the intercellular junctions of the brain-endothelial interface.