Comparison of four clinical specimen types for detection of influenza A and B viruses by optical immunoassay (FLU OIA test) and cell culture methods
Comparison of four clinical specimen types for detection of influenza A and B viruses by optical immunoassay (FLU OIA test) and cell culture methods
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DOI：
10.1128/jcm.37.12.3971-3974.1999
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发表时间：
1999-12-01
影响因子：
9.4
通讯作者：
Carlson, CA
中科院分区：
文献类型：
--
作者：
Covalciuc, KA;Webb, KH;Carlson, CA
虽然呼吸道病毒的实验室诊断已经得到了广泛的研究，但研究标本类型对甲型和乙型流感实验室诊断的影响的文献相对不足。在一项将流感OIA试验与L细胞培养进行比较的临床研究中，临床标本来自鼻咽拭子、喉咙拭子、鼻抽吸物和痰，这些标本来自出现流感样症状的患者。共收集184例患者的404份临床标本。如果任何样本地点的样本病毒培养呈阳性，患者被定义为流感阳性。如果所有样本点的样本病毒培养均为阴性，则患者被定义为流感阴性。根据这一金标准，对每种样本类型的培养和流感OIA测试结果进行了比较。对于四种样本类型中的每一种，病毒培养和流感OIA测试显示出同样的能力来检测甲型或乙型流感病毒或病毒抗原的存在。痰和鼻腔吸出物样本是最能预测流感病毒感染的样本。咽拭子是最不能预测流感病毒感染的，这两种测试都未能在近50%的研究喉咙样本中检测到流感病毒。
Although laboratory diagnosis of respiratory viruses has been widely studied, there is a relative insufficiency of literature examining the impact of specimen type on the laboratory diagnosis of influenza A and B. In a clinical study comparing the FLU OIA test with I l-day cell culture, clinical specimens from nasopharyngeal swabs, throat swabs, nasal aspirates, and sputum were obtained from patients experiencing influenza-like symptoms. A total of 404 clinical specimens were collected from 184 patients. Patients were defined as influenza positive if the viral culture of a specimen from any sample site was positive. Patients were defined as influenza negative if the viral cultures of specimens from all sample sites were negative. By this gold standard, culture and FLU OIA test results for each sample type were compared. For each of the four specimen types, the viral culture and FLU OIA test demonstrated equal abilities to detect the presence of influenza A or B virus or viral antigen. Sputum and nasal aspirate samples were the most predictive of influenza virus infection. Throat swabs were the least predictive of influenza virus infection, with both tests failing to detect influenza virus in nearly 50% of the throat samples studied.