Discovery of Anti-inflammatory Ingredients in Chinese Herbal Formula Kouyanqing Granule based on Relevance Analysis between Chemical Characters and Biological Effects.
Discovery of Anti-inflammatory Ingredients in Chinese Herbal Formula Kouyanqing Granule based on Relevance Analysis between Chemical Characters and Biological Effects.
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DOI：
10.1038/srep18080
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发表时间：
2015-12-10
影响因子：
4.6
通讯作者：
Su WW
中科院分区：
文献类型：
--
作者：
Liu H;Zheng YF;Li CY;Zheng YY;Wang DQ;Wu Z;Huang L;Wang YG;Li PB;Peng W;Su WW
口炎清颗粒是由金银花（FL）、玄参（RS）、麦冬（RO）、天冬（RA）、黄芪（RG）组成的传统中药复方。与传统的先分离再进行生物活性检测的方法不同，本研究旨在建立一种基于化学特征与生物活性相关性分析的方法，以确定KYQG抗炎作用的核心活性成分。通过改变5味中药的配比，制备了11个不同成分的KYQG样品。采用超快速液相色谱-二极管阵列检测器-四极杆-飞行时间串联质谱（UFLC-DAD-Q-TOF-MS/MS）技术鉴定了KYQG中的38种成分。将人口腔角质形成细胞（HOK）与5%香烟烟雾提取物（CSE）一起培养24小时以诱导炎症应激。用11个KYQG样品处理后，评价白细胞介素-1 β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）。灰色关联分析（GRA），皮尔逊相关（PCC），偏最小二乘法（PLS）被用来评估每个成分的贡献。结果表明，康养清颗粒可显著降低白细胞介素-1 β、白细胞介素-6、白细胞介素-8和肿瘤坏死因子-α的水平，其中赖氨酸、γ-氨基丁酸、白屈菜酸、酪氨酸、哈巴苷、新绿原酸、绿原酸、隐绿原酸、异槲皮苷、木犀草素-7-O-葡萄糖苷、3，4-二咖啡酰奎宁酸、3，5-二咖啡酰奎宁酸、安哥拉果苷C、哈巴苷、肉桂酸、和ruscogenin起着重要作用。
Kouyanqing Granule (KYQG) is a traditional Chinese herbal formula composed of Flos lonicerae (FL), Radix scrophulariae (RS), Radix ophiopogonis (RO), Radix asparagi (RA), and Radix et rhizoma glycyrrhizae (RG). In contrast with the typical method of separating and then biologicalily testing the components individually, this study was designed to establish an approach in order to define the core bioactive ingredients of the anti-inflammatory effects of KYQG based on the relevance analysis between chemical characters and biological effects. Eleven KYQG samples with different ingredients were prepared by changing the ratios of the 5 herbs. Thirty-eight ingredients in KYQG were identified using Ultra-fast liquid chromatography-Diode array detector-Quadrupole-Time-of-flight-Tandem mass spectrometry (UFLC-DAD-Q-TOF-MS/MS) technology. Human oral keratinocytes (HOK) were cultured for 24 hours with 5% of Cigarette smoke extract (CSE) to induce inflammation stress. Interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumour necrosis factor-α (TNF-α) were evaluated after treatment with the eleven KYQG samples. Grey relational analysis(GRA), Pearson’s correlations (PCC), and partial least-squares (PLS) were utilized to evaluate the contribution of each ingredient. The results indicated that KYQG significantly reduced interleukin-1β, interleukin-6, interleukin-8, and tumour necrosis factor-α levels, in which lysine, γ-aminobutyric acid, chelidonic acid, tyrosine, harpagide, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isoquercitrin, luteolin-7-o-glucoside, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, angoroside C, harpagoside, cinnamic acid, and ruscogenin play a vital role.