Assessing the role of p-cresol tolerance in Clostridium difficile
Assessing the role of p-cresol tolerance in Clostridium difficile
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DOI：
10.1099/jmm.0.47744-0
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发表时间：
2008-06-01
影响因子：
3
通讯作者：
Wren, Brendan W.
中科院分区：
文献类型：
--
作者：
Dawson, Lisa F.;Stabler, Richard A.;Wren, Brendan W.
艰难梭菌是一种重要的医院病原体，可导致抗生素相关疾病，从轻度腹泻到危及生命的伪膜性结肠炎。据信，接触抗生素后，患者的正常肠道微生物群被破坏，导致艰难梭菌增殖。值得注意的是，艰难梭菌是少数能够将酪氨酸发酵为对甲酚的细菌之一，对甲酚是一种酚类化合物，通过干扰新陈代谢的能力对其他微生物有毒。因此，不同艰难梭菌菌株产生和耐受对甲酚的能力可能在艰难梭菌相关疾病的发展和严重程度中发挥重要作用。在这项研究中，证明了两种艰难梭菌高毒力 027 菌株（Stoke Mandeville 和 BI-16）比其他艰难梭菌菌株（包括 630、CF4 和 CD196）对甲酚具有更高的耐受性。令人惊讶的是，索氏梭菌也对对甲酚具有高度耐受性，这表明这些梭菌物种的耐受途径存在重叠。
Clostridium difficile is an important nosocomial pathogen, resulting in antibiotic-associated disease ranging from mild diarrhoea to the life-threatening pseudomembranous colitis. Upon antibiotic exposure, it is believed that the normal bowel microflora of patients is disrupted, allowing C. difficile to proliferate. Significantly, C. difficile is among only a few bacteria able to ferment tyrosine to p-cresol, a phenolic compound that is toxic to other microbes via its ability to interfere with metabolism. Therefore, the ability of different C. difficile strains to produce and tolerate p-cresol may play an important role in the development and severity of C. difficile-associated disease. In this study, it was demonstrated that two C. difficile hypervirulent 027 strains (Stoke Mandeville and BI-16) are more tolerant to p-cresol than other C. difficile strains including 630, CF4 and CD196. Surprising, it was shown that Clostridium sordellii also has a high tolerance to p-cresol, suggesting an overlap in the tolerance pathways in these clostridial species.