Proteomics of DF-1 cells infected with avian leukosis virus subgroup J
Proteomics of DF-1 cells infected with avian leukosis virus subgroup J
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DOI：
10.1016/j.virusres.2012.05.016
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发表时间：
2012-08-01
期刊：
影响因子：
5
通讯作者：
Qin, Aijian
中科院分区：
文献类型：
--
作者：
Fan, Zhongjun;Hu, Xuming;Qin, Aijian
J亚群禽白血病病毒（ALV-J）是一种致癌性逆转录病毒，近年来给我国养禽业造成了严重的经济损失。病毒感染的发病机制和病毒与宿主的相互作用仍然没有很好地阐明。本文研究了ALV-J感染DF-1细胞后细胞内蛋白质的表达变化，比较分析发现，DF-1细胞内蛋白质的变化主要发生在ALV-J感染后6-12 h。质谱鉴定了74个改变的细胞蛋白，包括30个上调蛋白和44个下调蛋白。这些蛋白质中的一些参与细胞骨架、代谢过程、对刺激的反应和免疫反应。其他蛋白如DJ-1、UCHL 1、VDAC 1和HMGB 1与细胞凋亡或肿瘤发生也有一定的关系。通过实时RT-PCR证实了与未感染的DF-1细胞相比，感染的DF-1细胞中DJ-1、UCHL 1、VDAC 1和HMGB 1的转录谱的变化。我们的工作提供了ALV-J感染细胞中蛋白质表达差异的一些信息，这将有助于我们了解病毒的致病性。(c)2012爱思唯尔有限公司版权所有。
Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has led to severe economic losses in the poultry industry in China in recent years. The pathogenesis of virus infection and virus-host interactions are still not well elucidated. In this paper, we investigated the expression changes for cellular proteins in DF-1 cells infected with ALV-J. Comparative analyses revealed that the majority of the altered proteins in DF-1 cells appeared at 6-12 h after ALV-J infection. Mass spectrometry identified 74 altered cellular proteins, including 30 up-regulated proteins and 44 down-regulated proteins. Some of these proteins are involved in cell cytoskeleton, metabolic processes, response to stimulus and immune responses. Other proteins, such as DJ-1, UCHL1, VDAC1 and HMGB1, have some relationship to apoptosis or oncogenesis. The changes in the transcriptional profile of DJ-1, UCHL1, VDAC1 and HMGB1 in infected as compared to uninfected DF-1 cells were confirmed by real-time RT-PCR. Our work gives some information about differential protein expression in cells infected with ALV-J, which will help us to understand viral pathogenicity. (c) 2012 Elsevier B.V. All rights reserved.