Presence of a mannoprotein, MnpAp, in the hyphal cell wall of Aspergillus nidulans
Presence of a mannoprotein, MnpAp, in the hyphal cell wall of Aspergillus nidulans
复制标题
构巢曲霉菌丝细胞壁中存在甘露糖蛋白 MnpAp
DOI：
10.1080/15572536.2005.11832996
复制
发表时间：
2004
期刊：
影响因子：
2.8
通讯作者：
S. Whang
中科院分区：
文献类型：
--
作者：
H. Jeong;K. Chae;S. Whang
使用 mnpA 无效突变体作为阴性对照，通过免疫金电子显微镜检查构巢曲霉菌丝细胞壁中甘露糖蛋白 MnpAp 的存在。野生型的菌丝细胞壁由两层组成——电子致密的光滑外层和电子半透明的内层——而mnpA无效突变体的菌丝细胞壁具有电子致密的不规则外层和电子半透明的内层。在野生型中，MnpAp 存在于整个菌丝细胞壁的电子半透明层中，但不存在于分生孢子细胞壁中。在 mnpA 缺失突变体中，两种细胞类型的细胞壁均不存在 MnpAp。这些结果表明 MnpAp 存在于菌丝细胞壁中并且影响细胞壁表面结构。
The presence of a mannoprotein, MnpAp, in the hyphal cell wall of Aspergillus nidulans was examined by immunogold electron microscopy using a mnpA-null mutant as a negative control. The hyphal cell wall of wild type consisted of two layers—an electron-dense smooth outer layer and an electron-translucent inner layer—while the hyphal cell wall of the mnpA-null mutant had an electron-dense irregular outer layer together with the electron-translucent inner layer. In wild type, MnpAp was present throughout the electron-translucent layer of the hyphal cell wall but was absent from the conidial cell wall. In the mnpA-null mutant, MnpAp was absent from the cell walls of both cell types. These results indicate that MnpAp is present in the hyphal cell wall and that it influences cell wall surface structure.
构巢曲霉的 chsD 和 chsE 基因及其在几丁质合成中的作用。
DOI：
10.1006/fgbi.1996.0030
发表时间：
1996
期刊：
Fungal genetics and biology : FG & B.
影响因子：
--
作者：
Specht,CA;Liu,Y;Robbins,PW;Bulawa,CE;Iartchouk,N;Winter,KR;Riggle,PJ;Rhodes,JC;Dodge,CL;Culp,DW;Borgia,PT
通讯作者：
Borgia,PT