Comparison of Ribosomal DNA ITS Regions Among Hippophae rhamnoides ssp sinensis from Different Geographic Areas in China
Comparison of Ribosomal DNA ITS Regions Among Hippophae rhamnoides ssp sinensis from Different Geographic Areas in China
复制标题
中国不同地理区域沙棘核糖体DNA ITS区比较
DOI：
10.1007/s11105-010-0194-0
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发表时间：
2010-12-01
影响因子：
2.1
通讯作者：
Jin, Hai-peng
中科院分区：
文献类型：
--
作者：
Chen, Li-hong;Yu, Zhuo;Jin, Hai-peng
沙棘属沙棘属植物中华沙棘具有重要的生态和实用价值，利用rDNA ITS区的聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对沙棘的遗传多样性进行了研究。中国北部19个地点的中华按蚊。限制性内切酶分析将样品分为7个RFLP带型(I-VII)，表明沙棘属SSP。中华绒毛具有一定的遗传多样性。沙棘属植物的遗传多样性。华支睾吸虫主要分布在种群内，而不是种群间，地理距离对其遗传分化没有明显影响。RDNA ITS区序列长度在656~658bp之间，序列同源性为95%~100%。在这些沙棘SSP内的两两参数木村距离。华支睾吸虫样本在0.27%~5.95%之间。在整个ITS区有48个可变点，其中转换比颠换更频繁。沙棘ITS1和ITS2的转录本。中华绒毛具有保守的二级结构，这意味着ITS区域可能承受着维持参与rRNA转录后加工的RNA二级结构的进化压力。
Hippophae rhamnoides ssp. sinensis is of ecological and practical importance, PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the rDNA ITS region was conducted to estimate the genetic diversity of H. rhamnoides ssp. sinensis at 19 sites in Northern China. Restriction analysis divided the samples into seven RFLP patterns (I-VII), which implied that H. rhamnoides ssp. sinensis had a degree of genetic diversity. The genetic diversity of H. rhamnoides ssp. sinensis mainly lied within populations other than among populations, and the results also suggested that geographic distances did not have any clear effect on their genetic differentiation. The sequences of rDNA ITS region varied in length from 656 to 658 bp, which exhibited 95-100% sequence identity. Pairwise Kimura 2-parameter distances within these H. rhamnoides ssp. sinensis samples ranged from 0.27% to 5.95%. There were 48 variable sites within the entire ITS region, among them transitions were more frequent than transversions. The ITS1 and ITS2 transcripts of H. rhamnoides ssp. sinensis had conserved secondary structures, implying that the ITS region maybe under evolutionary pressure to maintain the RNA secondary structure involved in the post-transcriptional processing of rRNA.