Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: Suggestion for classification by immunophenotype and T-cell receptor studies
Heterogeneity of T-acute lymphoblastic leukemia (T-ALL) cell lines: Suggestion for classification by immunophenotype and T-cell receptor studies
复制标题
DOI：
10.1016/s0145-2126(98)00133-7
复制
发表时间：
1999-01-01
影响因子：
2.7
通讯作者：
Gramatzki, M
中科院分区：
文献类型：
--
作者：
Burger, R;Hansen-Hagge, TE;Gramatzki, M
造血细胞系通常用作某一细胞分化谱系和阶段的代表，特别是在免疫学和血液学研究中。T细胞型急性淋巴细胞白血病（ALL）至少在免疫表型上是一个相当异质的ALL组。我们的目的是提出一个全面的表征常用的T细胞白血病细胞系，并建议与正常分化模式的相关性。共分析了16个T-ALL细胞系的免疫表型和T细胞受体（TCR）重排和表达。20个细胞表面标志物的面板包括两个新的单克隆抗体（MoAb），TC-12和TH-111，这是在我们的实验室提出的，并检测T细胞ALL的亚群。在第五届人类白细胞分化抗原（HLDA）会议上，TC-12被分型为“独特的”，TH-111被分配到CD 96簇。我们根据欧洲白血病免疫学鉴定小组（EGIL）的最新建议将16个细胞系分为四组pro-T、pre-T、皮质T和成熟T分化阶段。有趣的是，没有发现T细胞系是相似的。总之，当使用T细胞白血病细胞系作为恶性或正常T细胞的模型时，似乎有必要考虑每个细胞系的特定分化阶段。(C)1998爱思唯尔科技有限公司版权所有。
Hematopoietic cell lines are often used as representatives for a certain cell differentiation lineage and stage, particularly in immunological and hematological studies. Acute lymphoblastic leukemia (ALL) of T-cell type is a rather heterogeneous group of ALL at least by immunophenotyping. Our aim was to present a comprehensive characterization of frequently used T-cell leukemia cell lines and to suggest a correlation with the normal differentiation pattern. A total of 16 T-ALL cell lines were analyzed for their immunophenotype and for T-cell receptor (TCR) rearrangement and expression. The panel of 20 cell surface markers included two new monoclonal antibodies (MoAb), TC-12 and TH-111, which were raised in our laboratory and detect subpopulations of T-cell ALL. TC-12 was typed 'unique', TH-111 was assigned to the CD96 cluster at the Vth Conference on human leucocyte differentiation antigens (HLDA). We categorized the 16 cell lines into the four groups pro-T, pre-T, cortical T and mature T differentiation stage according to the recent proposal of the European Group for the Immunological Characterization of Leukemias (EGIL). Interestingly, none of the T-cell lines were found to be alike. In conclusion, it appears necessary to consider the particular differentiation stage of each individual cell line when using T-cell leukemia lines as models for malignant or normal T cells. (C) 1998 Elsevier Science Ltd. All rights reserved.