Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa
Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa
复制标题
DOI：
10.1016/j.anireprosci.2013.03.016
复制
发表时间：
2013-06-01
影响因子：
2.2
通讯作者：
Ardabili, Farhad Farrokhi
中科院分区：
文献类型：
--
作者：
Ashrafi, Iraj;Kohram, Hamid;Ardabili, Farhad Farrokhi
在冷冻-解冻过程中产生的活性氧物种可能会降低精子质量。本研究评估了在精液稀释液中添加褪黑素作为抗氧化剂对公牛精子解冻后参数的影响。将褪黑素添加到柠檬酸-蛋黄延长剂中，得到六种不同的最终浓度：0，0.1，1，2，3和4 mM。精液是从6头经过验证的荷斯坦公牛身上采集的。精液在装在吸管中的稀释液中稀释，吸管用液氮冷冻。添加不同剂量褪黑素的精液稀释液冻融后精子总活动率、前向活动率、直线度、精子轨迹直线度、精子头部侧移、精子存活率、精子膜完整性和精子总体正常形态均显著提高(p<；0.05)。在显微镜下评价公牛精子冷冻延长器时，褪黑素的最有效浓度为2 mM。精液中添加3 mM褪黑素时，精液总抗氧化能力最高(p<；0.05)，脂质过氧化能力最低(2.7+/-0.8)，过氧化氢酶活性最高(0.7+/-0.1)。4毫摩尔浓度的褪黑素降低了前进运动和直线运动速度(p<；0.05)。综上所述，在精液稀释液中添加2~3 mM浓度的褪黑素可以改善解冻后精液的质量，这可能与减少脂质过氧化、提高总抗氧化能力和抗氧化酶活性有关。(C)2013爱思唯尔B.V.保留所有权利。
Reactive oxygen species generated during the freeze-thawing process may reduce sperm quality. This study evaluates the effects of melatonin supplementation as an antioxidant in the semen extender on post-thaw parameters of bull spermatozoa. Melatonin was added to the citrate-egg yolk extender to yield six different final concentrations: 0, 0.1, 1, 2, 3 and 4 mM. Ejaculates were collected from six proven Holstein bulls. Semen was diluted in the extender packaged in straws, which was frozen with liquid nitrogen. The semen extender supplemented with various doses of melatonin increased (p < 0.05) total motility, progressive motility, linearity, sperm track straightness, lateral head displacement, viability, integrity of the sperm membrane and total normal morphology of sperm after the freeze-thawing process. The most effective concentration of melatonin in microscopic evaluations of the bull sperm freezing extender was 2 mM. The highest (p < 0.05) value of total antioxidant capacity (48.9 +/- 2.7) and the lowest value of lipid peroxidation (2.7 +/- 0.8) were achieved by inclusion of 3 mM concentration of melatonin in the semen extender and the highest activity of catalase (0.7 +/- 0.1) was obtained by 2 mM melatonin. Four millimolar concentration of melatonin were reduced (p < 0.05) the progressive motility and straight linear velocity. In conclusion, supplementation of 2 or 3 mM concentration of melatonin in the semen extender improved the quality of post-thawed semen, which may associate with a reduction in lipid peroxidation as well as an increase in the total antioxidant capacity and antioxidant enzyme activity. (c) 2013 Elsevier B.V. All rights reserved.