Polyvinylpyrrolidone (PvP) adsorbs on and interacts with biomembrane-like layers
Polyvinylpyrrolidone (PvP) adsorbs on and interacts with biomembrane-like layers
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DOI：
10.1007/s10008-023-05784-4
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发表时间：
2023-12-28
影响因子：
2.5
通讯作者：
Nelson，Andrew
中科院分区：
文献类型：
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作者：
Crow，Bethany;Stokes，William E.;Nelson，Andrew
本文介绍了聚乙烯吡咯烷酮（PVP）溶液与二油酰磷脂酰胆碱（DOPC）电极支持单分子膜相互作用的初步研究。实验在pH 7.4的磷酸盐缓冲盐水（PBS）中进行，并且在流动池中的DOPC涂覆的微制造Hg/Pt电极上筛选溶液。在40 Vs−1下记录PvP相互作用对快速循环伏安图（RCV）形式的影响。发现PvP-DOPC相互作用强烈依赖于PvP链长。对于较短的链长，相互作用是线性相关的PvP浓度，而在较长的链长，相互作用是朗缪尔;然而，在所有情况下的相互作用RCV是吸附的代表。从这些图中提取亲和常数K2和检测限（LoD），这些值彼此呈负相关。logK 2和-log LoD与单体链段数（PvPm）的曲线拟合由幂项和指数项组成的双项方程。（logK 2）/PvPm与PvPmare的曲线图接近倒数，表明在吸附过程中DOPC表面上的链存在“成环”。结果符合熵驱动的吸附在短链长和在较长的链长，后者假定由于非共价相互作用的PvP链之间的DOPC表面上的吸附驱动的吸附模型。
This communication describes an initial study into the interaction of solution polyvinylpyrrolidone (PvP) with electrode-supported monolayers of dioleoyl phosphatidylcholine (DOPC). Experiments were carried out in phosphate buffered saline (PBS) at pH 7.4, and solutions were screened on a DOPC-coated microfabricated Hg/Pt electrode in flow cell. The effect of the PvP interaction on the form of rapid cyclic voltammograms (RCV) was recorded at 40 Vs−1. It was found that the PvP-DOPC interaction is strongly dependent on PvP chain length. For shorter chain lengths, the interaction is linearly related to PvP concentration whereas at longer chain lengths, the interaction is Langmuirean; however, the interaction RCV in all cases is representative of adsorption. Both the affinity constant,K2, and the limit of detection (LoD) are extracted from these plots, and these values are inversely related to each other. Plots of logK2and –log LoD versus the monomer segment number (PvPm) fit a two-term equation consisting of a power term and an exponential term. Plots of (logK2)/PvPmversus PvPmare near reciprocal showing that there is ‘looping’ of the chains on the DOPC surface during the adsorption process. The results fit a model of entropically driven adsorption at short chain lengths and enthalpically driven adsorption at longer chain lengths the latter assumedly due to non-covalent interactions between the PvP chains on the DOPC surface.