The inhibitory effect Polygonum Cillinerve polysaccharide on transmissible gastroenteritis virus of swine
The inhibitory effect Polygonum Cillinerve polysaccharide on transmissible gastroenteritis virus of swine
复制标题
何首乌多糖对猪传染性胃肠炎病毒的抑制作用
DOI：
10.1016/j.rvsc.2021.08.005
复制
发表时间：
2021-08-13
影响因子：
2.4
通讯作者：
Fan, Yunpeng
中科院分区：
文献类型：
--
作者：
Pan, Xingxue;Zhou, Yu;Fan, Yunpeng
猪传染性胃肠炎病毒（TGEV）是引起猪病毒性腹泻的主要病原体之一。本研究研究了蓼多糖（PCP）对TGEV的抑制作用。首先采用MTT法测定PCP细胞活力；采用Hoechst 33258荧光染色、Annexin V-FITC/PI荧光染色、real-time PCR和western blot检测PCP对TGEV的抑制作用。结果表明，PCP能显著降低TGEV诱导的细胞凋亡率，降低ROS的表达，减少TGEV的复制，增加Bcl-2和Bax基因的表达水平，增加Bcl-2蛋白的表达，降低Cyto c蛋白的表达，减少caspase 3的裂解量。因此，PCP对TGEV有较好的抑制作用，为TGEV的防治提供了一定的理论依据。
Transmissible gastroenteritis virus of swine (TGEV) is one kind of the main pathogens causing viral diarrhea in pig. In this study, the inhibitory effect of Polygonum Cillinerve polysaccharide (PCP) on TGEV was studied. Firstly, MTT method was used to measure the cell viability of PCP. Then Hoechst 33258 fluorescence staining, Annexin V-FITC/PI fluorescence staining, real-time PCR and western blot were used to explore the effect of PCP on inhibiting TGEV. The results showed that PCP could significantly reduce the apoptosis rate induced by TGEV, reduce the expression of ROS, reduce TGEV replication, increase the expression levels of Bcl-2 and Bax genes, increase the expression of Bcl-2 protein, decreased the expression of Cyto c protein, and reduce the amount of cleaved caspase 3. Therefore, PCP had the better inhibitory effect on TGEV, which provided a certain theoretical basis for the prevention and treatment of TGEV.