Diversity of foliar endophytes in wind-fallen Picea abies trees
Diversity of foliar endophytes in wind-fallen Picea abies trees
复制标题
DOI：
10.1007/s13225-011-0112-2
复制
发表时间：
2012-05-01
期刊：
影响因子：
20.3
通讯作者：
Baldrian, Petr
中科院分区：
文献类型：
--
作者：
Koukol, Ondrej;Kolarik, Miroslav;Baldrian, Petr
波希米亚森林国家公园（捷克共和国）内未管理的挪威云杉山地森林在过去十年中多次发生大规模的树皮甲虫爆发。本研究对该地区11株新近被风吹倒的树木针叶内生真菌多样性进行了调查。我们的目的是描述它们的多样性，并确定孤立的内生菌物种的相对宿主和器官特异性，以估计物种库和丰富的叶面内生菌在森林退化之前。从表面消毒的针叶中分离出真菌，通过形态学和分子生物学特征（ITS1、ITS2和部分28SrDNA）鉴定其生长菌株。针叶中的真菌群落是多样的，子囊菌（主要是无性型的Helotiales）占主导地位的担子菌。最常见的物种（sp.，，sp.和2种）与以往spp.例如，在这项研究中很少记录广泛分布的。这种格局可能是由不同的消毒和栽培方法或针叶的生理特性引起的，也可能反映了研究区的物种分布。成员的Helotiales，沿着与序列从GenBank中，显示出大量的重叠，在主机的亲和力，最显着的和之间，也从遥远的植物谱系的物种。
The unmanaged Norway spruce montane forests in the Bohemian Forest National Park (Czech Republic) suffered from repeated large-scale bark beetle outbreaks in last decade. In this study, the diversity of culturable foliar endophyte microfungi in needles originating from eleven recently wind-fallen trees in this area was surveyed. Our aims were to describe their diversity and to determine the relative host and organ specificities of isolated endophyte species to estimate the species pool and abundance of foliar endophytes before the forest degradation. Microfungi were isolated from surface-sterilized needles, and the outgrowing strains were identified based on morphological and molecular characteristics (analyses of ITS1, ITS2 and partial 28S rDNA). Fungal communities in the needles were diverse, with ascomycetes (mostly anamorphs of Helotiales) dominating basidiomycetes. The most frequent species ( sp., , sp. and two species of ) did not correspond with those recorded in previous studies of spp. For example, the widely distributed was rarely recorded in this study. This pattern may be caused by different methods of sterilization and cultivation or by physiological characteristics of the needles, or it may reflect the species distribution in the studied area. Members of the Helotiales, along with sequences from GenBank, showed substantial overlap in host affinities, most prominently between and and also among species from distant plant lineages.