ATP induces the release of IL-1 from LPS-primed cells in vivo.
ATP induces the release of IL-1 from LPS-primed cells in vivo.
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ATP 诱导体内 LPS 引发的细胞释放 IL-1。
DOI：
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发表时间：
1995
影响因子：
4.4
通讯作者：
Ivan G. Otterness
中科院分区：
文献类型：
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作者：
Richard J. Griffiths;E. Stam;J. Downs;Ivan G. Otterness
小鼠巨噬细胞体外分泌IL-1是一个低效的过程，与IL-6等其他细胞因子的分泌过程不同。因此，我们在体内研究了这一过程，以确定这些差异是否保持不变。小鼠腹腔注射脂多糖可诱导细胞外液(腹腔灌洗)产生和释放IL-6。虽然细胞内IL-1α和IL-1β的诱导很容易被检测到，但这些细胞因子在细胞外没有被检测到。内毒素后2 h注射三磷酸腺苷可引起IL-1β、IL-1α、乳酸脱氢酶和β-N-乙酰氨基葡萄糖苷酶的迅速释放。Western印迹分析显示，很大一部分IL-1β以17 kDa形式释放，而IL-1α未被处理。腺苷5‘-O-(3-硫代三磷酸)也能引起IL-1的释放，但不能引起UTP或ADP。这表明，ATP介导的IL-1的释放是一种受体介导的现象，与细胞溶解有关。
The secretion of IL-1 from murine macrophages in vitro is an inefficient process that is distinct from those of other cytokines such as IL-6. We have therefore studied this process in vivo to see if these differences are maintained. Intraperitoneal injection of LPS in mice induced production and release of IL-6 into the extracellular fluid (peritoneal lavage). Although induction of intracellular IL-1 alpha and IL-1 beta was readily detected, these cytokines were not detected extracellularly. Injection of ATP 2 h after LPS led to the rapid extracellular release of IL-1 beta, IL-1 alpha, lactate dehydrogenase, and beta-N-acetylglucosaminidase. Western blot analysis revealed that a large proportion of the IL-1 beta was released as the 17-kDa form, whereas IL-1 alpha was unprocessed. Adenosine 5'-O-(3-thiotriphosphate) was also effective in causing IL-1 release but not UTP or ADP. This suggests that the ATP-mediated release of IL-1 is a receptor-mediated phenomenon that is associated with cell lysis.