Fas involvement in Ca(2+)-independent T cell-mediated cytotoxicity.
Fas involvement in Ca(2+)-independent T cell-mediated cytotoxicity.
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DOI：
10.1084/jem.177.1.195
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发表时间：
1993-01-01
期刊：
影响因子：
--
通讯作者：
Golstein P
中科院分区：
文献类型：
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作者：
Rouvier E;Luciani MF;Golstein P
T 细胞介导的细胞毒性机制在分子水平上仍不清楚。为了研究其中一些机制，我们一方面使用来自lpr和gld小鼠突变体的胸腺细胞，另一方面使用转染或未转染凋亡诱导Fas分子的L1210细胞作为靶细胞。这些独立的突变体或基于转染子的方法均得出这样的结论：Fas参与了由至少两种T细胞来源介导的不依赖Ca(2+)的细胞毒性成分，即非抗原特异性体外活化杂交瘤细胞和抗原特异性体内培养的腹膜渗出淋巴细胞。因此，在这些情况下，T 细胞介导的细胞毒性涉及通过 Fas 转导靶细胞死亡信号。
Mechanisms of T cell-mediated cytotoxicity remain poorly defined at the molecular level. To investigate some of these mechanisms, we used as target cells, on the one hand, thymocytes from lpr and gld mouse mutants, and on the other hand, L1210 cells transfected or not with the apoptosis-inducing Fas molecule. These independent mutant or transfectant-based approaches both led to the conclusion that Fas was involved in the Ca(2+)-independent component of cytotoxicity mediated by at least two sources of T cells, namely nonantigen-specific in vitro activated hybridoma cells, and antigen-specific in vivo raised peritoneal exudate lymphocytes. Thus, in these cases, T cell-mediated cytotoxicity involved transduction via Fas of the target cell death signal.