Effect of dose fractionation on the ethylnitrosourea induction of specific-locus mutations in mouse spermatogonia.
Effect of dose fractionation on the ethylnitrosourea induction of specific-locus mutations in mouse spermatogonia.
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DOI：
10.1073/pnas.79.11.3592
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发表时间：
1982-06
影响因子：
11.1
通讯作者：
W. Russell;P. Hunsicker;D. Carpenter;C. Cornett;G. M. Guinn
中科院分区：
文献类型：
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作者：
W. Russell;P. Hunsicker;D. Carpenter;C. Cornett;G. M. Guinn
根据小鼠精原细胞的特异性基因突变，将每公斤体重100毫克的乙基亚硝脲分成每周一次的10毫克/公斤的剂量，与单次注射100毫克/公斤相比，突变频率大大降低。由于有独立证据表明，10毫克/公斤和100毫克/公斤的剂量到达生殖细胞的数量与注射剂量成正比，因此分次剂量的突变反应较低被归因于修复。单次10毫克/公斤剂量的诱变率(假设这将是10次剂量后观察到的十分之一的诱变率)仅为自发突变率的75%。小鼠精原细胞显然有一种有效的修复系统，即使对强大的诱变剂也是有效的。
As measured by specific-locus mutations in mouse spermatogonia, fractionating a dose of 100 mg of ethylnitrosourea per kg of body weight into doses of 10 mg/kg injected intraperitoneally at weekly intervals greatly reduces the mutation frequency compared with that from a single dose of 100 mg/kg. Because there is independent evidence that the doses of 10 and 100 mg/kg reach the germ cells in amounts directly proportional to the injected dose, the lower mutational response with the fractionated dose is attributed to repair. The induced mutation rate expected from a single 10-mg/kg dose (on the assumption that this would be 1/10th the rate observed after 10 such doses) would be only 75% of the spontaneous mutation rate. Mouse spermatogonia apparently have an efficient repair system that is effective even against a potent mutagen.