Identification of Bartonella Infections in Febrile Human Patients from Thailand and Their Potential Animal Reservoirs
Identification of Bartonella Infections in Febrile Human Patients from Thailand and Their Potential Animal Reservoirs
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DOI：
10.4269/ajtmh.2010.09-0778
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Peruski, Leonard F.
中科院分区：
文献类型：
--
作者：
Kosoy, Michael;Bai, Ying;Peruski, Leonard F.
为了确定巴尔通体物种在泰国人类急性发热性疾病中的作用，我们采用了一种新策略，将血液与真核细胞共培养，并对巴尔通体特异性 DNA 产物进行系统发育分析。在发热患者的 14 个血凝块中鉴定出了巴尔通体。序列分析显示，在人类患者中鉴定的基因型中有一半以上与亚洲啮齿动物中鉴定的同源序列相似或相同，并且与 B. elizabethae、B.rattimassiliensis 和 B.tribocorum 密切相关。其余基因型属于 B. henselae、B. vinsonii 和 B. tamiae。在阳性发热患者中，动物接触很常见：36% 的人报告拥有狗或猫，71% 的人报告在发病前 2 周内接触过老鼠。研究结果表明，啮齿动物可能是泰国大部分人类巴尔通体感染病例的宿主。
To determine the role of Bartonella species as causes of acute febrile illness in humans from Thailand, we used a novel strategy of co-cultivation of blood with eukaryotic cells and subsequent phylogenetic analysis of Bartonella-specific DNA products. Bartonella species were identified in 14 blood clots from febrile patients. Sequence analysis showed that more than one-half of the genotypes identified in human patients were similar or identical to homologous sequences identified in rodents from Asia and were closely related to B. elizabethae, B. rattimassiliensis, and B. tribocorum. The remaining genotypes belonged to B. henselae, B. vinsonii, and B. tamiae. Among the positive febrile patients, animal exposure was common: 36% reported owning either dogs or cats and 71% reported rat exposure during the 2 weeks before illness onset. The findings suggest that rodents are likely reservoirs for a substantial portion of cases of human Bartonella infections in Thailand.