Collagen-gel-embedded three-dimensional culture of human thyroid epithelial cells: comparison between the floating sandwich method and the dispersed embedding method.
Collagen-gel-embedded three-dimensional culture of human thyroid epithelial cells: comparison between the floating sandwich method and the dispersed embedding method.
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人甲状腺上皮细胞胶原凝胶包埋三维培养：浮动夹心法与分散包埋法的比较
DOI：
10.1247/csf.16.209
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发表时间：
1991
影响因子：
1.5
通讯作者：
M. Mori
中科院分区：
文献类型：
--
作者：
M. Sasaki;N. Sawada;T. Minase;M. Satoh;M. Mori
用胶原酶从手术切除的人甲状腺中分离人甲状腺上皮细胞，并在补充egf的条件下培养一周，使其增殖。然后将细胞转移到以下三维培养系统中。其中一种方法是将分离细胞培养在漂浮的双层胶原凝胶之间，称为“漂浮三明治法”。另一种方法是将分离细胞与胶原蛋白凝胶混合培养，称为“分散包埋法”。用前一种方法获得了许多具有相当大小的腔体的滤泡样结构。用漂浮夹层法培养的细胞表现出明显的极性，在顶端表面存在大量微绒毛，在基底表面与胶原凝胶紧密接触。另一方面，分散包埋法只获得了少量的滤泡状结构，滤泡状结构体积较小，细胞的极性也不如漂浮夹心法明显。因此，漂浮夹层法似乎适合于研究人甲状腺上皮细胞体外组织滤泡结构的过程和机制。
Human thyroid epithelial cells were isolated from surgically resected human thyroid gland with collagenase and cultured for one week under EGF-supplemented conditions to allow them to proliferate. Then the cells were transferred to the following three-dimensional culture systems. One was a culture of isolated cells between floating double layers of collagen gel, designated the "floating sandwich method." The other was a culture of isolated cells mixed with collagen gel, designated the "dispersed embedding method." Many folliclelike structures with lumina of appreciable size were obtained by the former method. The cells cultured by the floating sandwich method exhibited a distinct polarity shown by the presence of numerous microvilli at the apical surface and close contact with collagen gels at the basal surface. On the other hand, only a few folliclelike structures were obtained by the dispersed embedding method, in which the folliclelike structures were small in size and the cells showed less distinct polarity than those observed in the floating sandwich method. Thus, the floating sandwich method appears to be suitable for studying the process and mechanism of in vitro organization of follicular structures by human thyroid epithelial cells.